Novel Stress-Inducible Antisense RNAs of Protein-Coding Loci Are Synthesized by RNA-Dependent RNA Polymerase1[OPEN]
Novel Stress-Inducible Antisense RNAs of Protein-Coding Loci Are Synthesized by RNA-Dependent RNA Polymerase1[OPEN]
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RNA 依赖性 RNA 聚合酶合成蛋白质编码位点的新型应激诱导反义 RNA1[打开]
作者：
A. Matsui;K. Iida;Maho Tanaka;K. Yamaguchi;Kayoko Mizuhashi;Jong;S. Takahashi;Norio Kobayashi;S. Shigenobu;K. Shinozaki;M. Seki
由RNA依赖的RNA聚合酶产生的应激诱导的非编码反义RNA在一种不同于已知内源性小RNA途径的新的非生物应激反应机制中起作用。我们之前的研究发现了大约6000个存在于蛋白质编码基因反义链上的非生物应激应答非编码转录本，并暗示了一种反义RNA是由RNA依赖的RNA聚合酶（RDR）从一个义RNA模板合成的。表达分析显示，rdr1/2/6突变体中1136个基因位点上新的非生物胁迫诱导的反义RNA表达减少。RNase保护表明，RD29A反义RNA和其他依赖rdr1 /2/6的反义RNA参与了dsRNA的形成。胁迫诱导的反义RNA的积累在dcp5和xrn4中分别减少和增加，而在dcl2/3/4、nrpd1a和nrpd1b中没有变化。RNA-seq分析显示，大多数依赖rdr1 /2/6的反义RNA位点与依赖rdr1 /2/6的20 - 30nt RNA位点不重叠。此外，rdr1/2/6突变体降低了感觉RNA的降解速率，在干旱胁迫后的恢复阶段表现出根系生长停滞，而dcl2/3/4突变体则没有。总之，这些结果表明rdr具有应激诱导的反义RNA合成活性和不同于已知内源性小RNA途径的新的生物学功能。这些数据揭示了非生物应激反应中RNA调控的新机制，涉及复杂的RNA降解途径。
Stress-inducible non-coding antisense RNAs generated by RNA-dependent RNA polymerases function in a novel abiotic stress response mechanism different from the known endogenous small RNA pathways. Our previous study identified approximately 6,000 abiotic stress-responsive noncoding transcripts existing on the antisense strand of protein-coding genes and implied that a type of antisense RNA was synthesized from a sense RNA template by RNA-dependent RNA polymerase (RDR). Expression analyses revealed that the expression of novel abiotic stress-induced antisense RNA on 1,136 gene loci was reduced in the rdr1/2/6 mutants. RNase protection indicated that the RD29A antisense RNA and other RDR1/2/6-dependent antisense RNAs are involved in the formation of dsRNA. The accumulation of stress-inducible antisense RNA was decreased and increased in dcp5 and xrn4, respectively, but not changed in dcl2/3/4, nrpd1a and nrpd1b. RNA-seq analyses revealed that the majority of the RDR1/2/6-dependent antisense RNA loci did not overlap with RDR1/2/6-dependent 20–30 nt RNA loci. Additionally, rdr1/2/6 mutants decreased the degradation rate of the sense RNA and exhibited arrested root growth during the recovery stage following a drought stress, whereas dcl2/3/4 mutants did not. Collectively, these results indicate that RDRs have stress-inducible antisense RNA synthesis activity and a novel biological function that is different from the known endogenous small RNA pathways from protein-coding genes. These data reveal a novel mechanism of RNA regulation during abiotic stress response that involves complex RNA degradation pathways.
影响因子：
10.5
作者：
Yoshikawa, M;Peragine, A;Poethig, RS
通讯作者：
Poethig, RS
影响因子：
64.5
作者：
Autran, Daphne;Baroux, Celia;Grossniklaus, Ueli
通讯作者：
Grossniklaus, Ueli
影响因子：
4.5
作者：
Gu, Rui;Zhang, Zuo;Carmichael, Gordon G.
通讯作者：
Carmichael, Gordon G.