Endoplasmic Reticulum-Localized PURINE PERMEASE1 Regulates Plant Height and Grain Weight by Modulating Cytokinin Distribution in Rice.
Endoplasmic Reticulum-Localized PURINE PERMEASE1 Regulates Plant Height and Grain Weight by Modulating Cytokinin Distribution in Rice.
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DOI：
10.3389/fpls.2020.618560
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发表时间：
2020
影响因子：
5.6
通讯作者：
Tang W
中科院分区：
文献类型：
--
作者：
Xiao Y;Zhang J;Yu G;Lu X;Mei W;Deng H;Zhang G;Chen G;Chu C;Tong H;Tang W
细胞分裂素（Cytokinins, ck）是一类在多种生物过程中起重要作用的植物激素。然而，CK转运的机制及其在植物生长发育中的作用还远未完全阐明。在此，我们研究了PURINE PERMEASE1 （OsPUP1）在水稻中的功能。OsPUP1主要在根中表达，尤其是在维管细胞中，CK处理可以诱导其表达。亚细胞定位分析表明OsPUP1主要定位于内质网（ER）。OsPUP1过表达导致地上组织生长缺陷，包括叶长、株高、粒重、穗长和粒数减少。激素分析结果显示，与野生型相比，过表达ospup1的幼苗茎部CK含量降低，根部CK含量增加。穗部CK含量也降低。以多个CK - a型响应调控因子（osrr）为标记基因的定量反转录pcr （qRT-PCR）分析表明，当CK施于根系时，过表达ospup1的幼苗的茎部CK响应较野生型降低。遗传分析表明，BG3/OsPUP4是一种被推测为质膜定位的CK转运蛋白，可以克服OsPUP1的功能。我们假设OsPUP1可能参与将ck导入内质网，并通过细胞间转运将ck从维管组织中卸下。
Cytokinins (CKs) are a class of phytohormones playing essential roles in various biological processes. However, the mechanisms underlying CK transport as well as its function in plant growth and development are far from being fully elucidated. Here, we characterize the function of PURINE PERMEASE1 (OsPUP1) in rice (Oryza sativa L.). OsPUP1 was predominantly expressed in the root, particularly in vascular cells, and CK treatment can induce its expression. Subcellular localization analysis showed that OsPUP1 was predominantly localized to the endoplasmic reticulum (ER). Overexpression of OsPUP1 resulted in growth defect of various aerial tissues, including decreased leaf length, plant height, grain weight, panicle length, and grain number. Hormone profiling revealed that the CK content was decreased in the shoot of OsPUP1-overexpressing seedling, but increased in the root, compared with the wild type. The CK content in the panicle was also decreased. Quantitative reverse transcription-PCR (qRT-PCR) analysis using several CK type-A response regulators (OsRRs) as the marker genes suggested that the CK response in the shoot of OsPUP1-overexpressing seedling is decreased compared to the wild type when CKs are applied to the root. Genetic analysis revealed that BG3/OsPUP4, a putative plasma membrane-localized CK transporter, overcomes the function of OsPUP1. We hypothesize that OsPUP1 might be involved in importing CKs into ER to unload CKs from the vascular tissues by cell-to-cell transport.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
6.9
作者：
Caesar K;Thamm AM;Witthöft J;Elgass K;Huppenberger P;Grefen C;Horak J;Harter K
通讯作者：
Harter K
影响因子：
7.4
作者：
Hirose, N;Makita, N;Sakakibara, H
通讯作者：
Sakakibara, H
影响因子：
1.6
作者：
Kakegawa, Hirofumi;Shitan, Nobukazu;Sugiyama, Akifumi
通讯作者：
Sugiyama, Akifumi
影响因子：
11.6
作者：
Hutchison, Claire E.;Li, Jie;Kieber, Joseph J.
通讯作者：
Kieber, Joseph J.