Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum.

Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum.
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DOI:
10.1093/jxb/err238
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发表时间:
2011-11
影响因子:
6.9
通讯作者:
Harter K
Harter K
中科院分区:
生物学1区
文献类型:
--
作者:
Caesar K;Thamm AM;Witthöft J;Elgass K;Huppenberger P;Grefen C;Horak J;Harter K

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细胞分裂素是一种激素,参与植物生长发育的各种过程。细胞分裂素的信号模式始于通过膜上定位的组氨酸激酶受体感受激素。虽然这些受体的生化特性和功能已经得到了广泛的研究,但还没有确凿的证据证明它们的亚细胞定位。在这里,提供了细胞分裂素受体家族成员拟南芥组氨酸激酶3(AHK3)和4(AHK4)的功能性荧光标记融合在内质网(ER)中定位的细胞生物学和生化证据。此外,膜结合的AHK3在体内与AHK4相互作用。内质网中细胞分裂素受体的定位和可能的功能对植物中细胞分裂素的感知和信号传递以及激素的串扰有着重要的影响。
Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.
DOI: 10.1093/bioinformatics/btq115
发表时间: 2010-05-01
期刊: Bioinformatics (Oxford, England)
影响因子: --
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