Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis.
Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis.
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DOI：
10.1016/j.bbadis.2008.08.011
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发表时间：
2008-11
影响因子：
6.2
通讯作者：
Kang, Jing X.
中科院分区：
文献类型：
--
作者：
Weylandt, Karsten H.;Nadolny, Anja;Kahlke, Lena;Koehnke, Thomas;Schmoecker, Christoph;Wang, Jingdong;Lauwers, Gregory Y.;Glickman, Jonathan N.;Kang, Jing X.
胰腺炎是一种发病率和死亡率都很高的严重致衰性疾病。治疗大多是支持性的，到目前为止还没有临床上有用的抗炎治疗策略。虽然omega-3多不饱和脂肪酸(n-3 PUFA)已知具有抗炎作用，但这些脂肪酸在减轻胰腺炎方面的效用仍有待研究。这项研究的目的是在一个良好控制的实验系统中检查n-3多不饱和脂肪酸对急性和慢性胰腺炎的影响。我们使用了以组织内源性n-3PUFA水平升高为特征的FAT-1转基因小鼠模型及其野生型窝种，以检测n-3PUFA对急性和慢性蓝蛋白诱导的胰腺炎的影响。通过组织学和分子生物学方法评估疾病活动性和炎症状态。在急性胰腺炎中，FAT-1小鼠表现出坏死减少的趋势，血浆IL-6水平显著降低，肺内中性粒细胞浸润减少。在慢性胰腺炎中，与组织中omega-6(n-6)PUFA水平高的野生型窝鼠相比，组织中n-3PUFA水平增加的FAT-1动物胰腺纤维化和胶原含量较少，并伴有胰腺星状细胞活性下降。我们的数据为通过增加omega-3多不饱和脂肪酸的组织含量来减少急性胰腺炎的全身炎症和慢性胰腺炎的组织纤维化提供了证据。这些结果表明n-3多不饱和脂肪酸在急性和慢性胰腺炎中的补充具有有益的潜力。
Pancreatitis is a severe debilitating disease with high morbidity and mortality. Treatment is mostly supportive, and until now there are no clinically useful strategies for anti-inflammatory therapy. Although omega-3 polyunsaturated fatty acids (n-3 PUFA) are known to have anti-inflammatory effects, the utility of these fatty acids in the alleviation of pancreatitis remained to be investigated. The aim of this study was to examine the effect of n-3 PUFA on both acute and chronic pancreatitis in a well-controlled experimental system. We used the fat-1 transgenic mouse model, characterised by endogenously increased tissue levels of n-3 PUFA, and their wild-type littermates to examine the effect of n-3 PUFA on both acute and chronic cerulein induced pancreatitis. Disease activity and inflammatory status were assessed by both histology and molecular methods. In acute pancreatitis, fat-1 mice showed a trend towards decreased necrosis and significantly reduced levels of plasma IL-6 levels as well as reduced neutrophil infiltration in the lung. In chronic pancreatitis there was less pancreatic fibrosis and collagen content accompanied by decreased pancreatic stellate cell activation in the fat-1 animals with increased n-3 PUFA tissue levels as compared to wild-type littermates with high levels of omega-6 (n-6) PUFA in their tissues. Our data provide evidence for a reduction of systemic inflammation in acute pancreatitis and of tissue fibrosis in chronic pancreatitis by increasing the tissue content of omega-3 polyunsaturated fatty acids. These results suggest a beneficial potential for n-3 PUFA supplementation in acute and particularly chronic pancreatitis.
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影响因子：
2.9
作者：
Okamoto, T;Yamada, T;Itoh, M
通讯作者：
Itoh, M
影响因子：
6.3
作者：
Lasztity, N;Hamvas, J;Antal, M
通讯作者：
Antal, M
DOI：
10.1016/j.ijsu.2008.01.003
发表时间：
2008-04-01
期刊：
International journal of surgery (London, England)
影响因子：
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作者：
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通讯作者：
Zagainov, Vladimir E
影响因子：
3.4
作者：
Foitzik, T;Eibl, G;Buhr, HJ
通讯作者：
Buhr, HJ
影响因子：
4.8
作者：
Fitzner, Brit;Brock, Peter;Jaster, Robert
通讯作者：
Jaster, Robert