Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription
Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription
复制标题
γδ解离酶基因的核苷酸序列及其基因产物作为转录阻遏蛋白的证明
作者：
R. Reed;G. I. Shibuya;J. Steitz
相关的转座子γδ和Tn 3各自编码转座所必需的有效位点特异性重组系统1，2。重组发生在一个特定的位点，res，位于转座所需的两个不同转录基因之间的顺反子间区域。这些基因之一，resolvase，催化重组在保留最近，在体外位点特异性重组已被证明使用纯化的resolvase和模型基板含有两个ressites 3。对编码解离酶蛋白的γδ/Tn 3 tnpR基因中的突变体的遗传分析表明，解离酶除了催化重组之外还调节转座的频率4 -6。元素编码的蛋白质在微细胞中的分析表明，解离酶的元素编码的转座酶的水平上的直接影响，以及对自己的表达7。本文报道了编码γδ解离酶的基因序列，并将预测的氨基酸序列与功能可互换的Tn 3蛋白6的氨基酸序列进行了比较。此外，我们已经确定了转座酶和解离酶基因在体外启动转录的位点，并证明了纯化的解离酶蛋白特异性地抑制这两个转座相关基因在体外转录系统中的转录。
The related transposons γδ and Tn3 each encode an efficient site-specific recombination system essential for transposition1,2. The recombination occurs at a particular site, res, located within the intercistronic region between the two divergently transcribed genes that are required for transposition2. The product of one of these genes, resolvase, catalyses the recombination at res. Recently, in vitro site-specific recombination has been demonstrated using purified resolvase and a model substrate containing two res sites3. Genetic analysis of mutants in the γδ/Tn3 tnpR gene, which encodes the resolvase protein, suggested that resolvase regulates the frequency of transposition in addition to catalysing recombination4–6. Analysis of element-encoded proteins in minicells indicated a direct effect of resolvase on the levels of the element-encoded transposase, as well as on its own expression7. Here we report the sequence of the gene encoding γδ resolvase and compare the predicted amino acid sequence with that of the functionally interchangeable Tn3 protein6. In addition, we have determined the sites at which transcription of the transposase and resolvase genes initiates in vitro and have demonstrated that purified resolvase protein specifically represses transcription of both transposition related genes in this in vitro transcription system.
DOI：
10.1073/pnas.78.2.752
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LEE, NL;GIELOW, WO;WALLACE, RG
通讯作者：
WALLACE, RG
转座子指定的位点特异性重组。
DOI：
10.1073/pnas.79.1.46
发表时间：
1982
影响因子：
11.1
作者：
Kitts,P;Symington,L;Burke,M;Reed,R;Sherratt,D
通讯作者：
Sherratt,D
转座子 γ δ 和 Tn3 之间的共整合的解析定义了重组位点。
DOI：
10.1073/pnas.78.6.3428
发表时间：
1981
影响因子：
11.1
作者：
Reed,RR
通讯作者：
Reed,RR