Immunization of mice with recombinant protein CobB or AsnC confers protection against Brucella abortus infection.
Immunization of mice with recombinant protein CobB or AsnC confers protection against Brucella abortus infection.
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用重组蛋白 CobB 或 AsnC 免疫小鼠可预防流产布鲁氏菌感染
DOI：
10.1371/journal.pone.0029552
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Fu S;Xu J;Li X;Xie Y;Qiu Y;Du X;Yu S;Bai Y;Chen Y;Wang T;Wang Z;Yu Y;Peng G;Huang K;Huang L;Wang Y;Chen Z
由于减毒活疫苗的缺陷，布鲁氏菌保护性抗原作为亚单位疫苗候选者的筛选受到了越来越多的关注。布鲁氏菌是一种兼性胞内细菌，细胞免疫在预防布鲁氏菌感染中起着至关重要的作用。识别向宿主呈递T细胞表位的布鲁氏菌抗原可以使此类疫苗的开发成为可能。在这项研究中，根据现有的数据，选择了45个已证实或可能与布鲁氏菌发病相关的因素。在表达和纯化后，35个蛋白符合体外刺激T细胞反应能力的分析。然后，用体外干扰素-γ试验鉴定流产杆菌的潜在T细胞抗原。从流产杆菌活疫苗S19免疫的小鼠脾细胞中，共鉴定出7种刺激干扰素-γ反应的蛋白。进一步评价了这7种重组蛋白的保护效果。给予BAB1_1316(Cobb)或BAB1_1688(ASNC)加佐剂的小鼠可对强毒流产鼠提供保护，与已知的保护性抗原铜锌超氧化物歧化酶和许可证疫苗S19相似。此外，Cobb和ASNC均能诱导BALB/c小鼠产生较强的抗体反应。综上所述，本研究表明Cobb或ASNC蛋白可作为布鲁氏菌病亚单位疫苗的候选抗原，具有足够的免疫原性和保护效力。
Due to drawbacks of live attenuated vaccines, much more attention has been focused on screening of Brucella protective antigens as subunit vaccine candidates. Brucella is a facultative intracellular bacterium and cell mediated immunity plays essential roles for protection against Brucella infection. Identification of Brucella antigens that present T-cell epitopes to the host could enable development of such vaccines. In this study, 45 proven or putative pathogenesis-associated factors of Brucella were selected according to currently available data. After expressed and purified, 35 proteins were qualified for analysis of their abilities to stimulate T-cell responses in vitro. Then, an in vitro gamma interferon (IFN-γ) assay was used to identify potential T-cell antigens from B. abortus. In total, 7 individual proteins that stimulated strong IFN-γ responses in splenocytes from mice immunized with B. abortus live vaccine S19 were identified. The protective efficiencies of these 7 recombinant proteins were further evaluated. Mice given BAB1_1316 (CobB) or BAB1_1688 (AsnC) plus adjuvant could provide protection against virulent B. abortus infection, similarly with the known protective antigen Cu-Zn SOD and the license vaccine S19. In addition, CobB and AsnC could induce strong antibodies responses in BALB/c mice. Altogether, the present study showed that CobB or AsnC protein could be useful antigen candidates for the development of subunit vaccines against brucellosis with adequate immunogenicity and protection efficacy.
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