PIPKs are essential for rhizoid elongation and caulonemal cell development in the moss Physcomitrella patens.
PIPKs are essential for rhizoid elongation and caulonemal cell development in the moss Physcomitrella patens.
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PIPK 对小立碗藓 (Physcomitrella patens) 中的根茎伸长和茎细胞发育至关重要
DOI：
10.1111/j.1365-313x.2011.04623.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Sommarin M
中科院分区：
文献类型：
--
作者：
Saavedra L;Balbi V;Lerche J;Mikami K;Heilmann I;Sommarin M
PtdIns‐4,5‐bisphosphate is a lipid messenger of eukaryotic cells that plays a critical role in processes such as cytoskeleton organization, intracellular vesicular trafficking, secretion, cell motility, regulation of ion channels and nuclear signalling pathways. The enzymes responsible for the synthesis of PtdIns(4,5)P2are phosphatidylinositol phosphate kinases (PIPKs). The mossPhyscomitrella patenscontains two PIPKs, PpPIPK1 and PpPIPK2. To study their physiological role, both genes were disrupted by targeted homologous recombination and as a result mutant plants with lower PtdIns(4,5)P2levels were obtained. A strong phenotype forpipk1, but not forpipk2single knockout lines, was obtained. Thepipk1knockout lines were impaired in rhizoid and caulonemal cell elongation, whereaspipk1‐2double knockout lines showed dramatic defects in protonemal and gametophore morphology manifested by the absence of rapidly elongating caulonemal cells in the protonemal tissue, leafy gametophores with very short rhizoids, and loss of sporophyte production.pipk1complemented by overexpression ofPpPIPK1fully restored the wild‐type phenotype whereas overexpression of the inactive PpPIPK1E885A did not. Overexpression ofPpPIPK2in thepipk1‐2double knockout did not restore the wild‐type phenotype demonstrating thatPpPIPK1andPpPIPK2are not functionally redundant.In vivoimaging of the cytoskeleton network revealed that the shortened caulonemal cells in thepipk1mutants was the result of the absence of the apicobasal gradient of cortical F‐actin cables normally observed in wild‐type caulonemal cells. Our data indicate that both PpPIPKs play a crucial role in the development of the mossP. patens, and particularly in the regulation of tip growth.
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DOI：
10.1105/tpc.9.7.1099
发表时间：
1997
期刊：
The Plant cell
影响因子：
--
作者：
K. Schumaker;M. Dietrich
通讯作者：
M. Dietrich
影响因子：
7.2
作者：
Schaefer, DG;Zryd, JP
通讯作者：
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影响因子：
9.4
作者：
Finka, Andrija;Schaefer, Didier G.;Zryd, Jean-Pierre
通讯作者：
Zryd, Jean-Pierre
影响因子：
56.9
作者：
Rensing, Stefan A.;Lang, Daniel;Boore, Jeffrey L.
通讯作者：
Boore, Jeffrey L.
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DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
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通讯作者：
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