Genomic islands from five strains of Burkholderia pseudomallei.
Genomic islands from five strains of Burkholderia pseudomallei.
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DOI：
10.1186/1471-2164-9-566
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发表时间：
2008-11-27
期刊：
影响因子：
4.4
通讯作者：
Keim P
中科院分区：
文献类型：
--
作者：
Tuanyok A;Leadem BR;Auerbach RK;Beckstrom-Sternberg SM;Beckstrom-Sternberg JS;Mayo M;Wuthiekanun V;Brettin TS;Nierman WC;Peacock SJ;Currie BJ;Wagner DM;Keim P
类鼻疽伯克霍尔德氏菌是类鼻疽病的病原体，在这种感染流行的地方是发病率和死亡率的重要原因。B菌株间的基因组差异。假鼻疽被预测为在类鼻疽病患者中观察到的多种临床表现的主要原因之一。本研究的目的是研究的作用，基因组岛（GI）的基因组多样性在这个物种的来源。我们发现，基因组岛（GI）之间的差异很大B。类鼻疽菌株。我们从B的5个参考菌株的基因组序列中鉴定出71个不同的GI。假鼻疽：K96243、1710 b、1106 a、MSHR 668和MSHR 305。这些GI的基因组位置不是随机的，因为它们中的许多与tRNA基因座相关。特别地，预测tRNA基因的3'末端序列参与GI的整合。我们提出的术语“tRNA介导的位点特异性重组”（tRNA-SSR）的这种机制。此外，我们提供了一个GI命名法，这是基于整合热点确定这里或以前描述。我们的数据表明，收购的地理标志是B内的基因组多样性的主要来源之一。类鼻疽和促进水平获得性GI的分子机制在B的多种菌株中是共同的。假鼻疽多个菌株中71个GI的差异存在证明了这些移动的元件对于塑造该细菌种属内单个菌株和群体的遗传组成的重要性。
Burkholderia pseudomallei is the etiologic agent of melioidosis, a significant cause of morbidity and mortality where this infection is endemic. Genomic differences among strains of B. pseudomallei are predicted to be one of the major causes of the diverse clinical manifestations observed among patients with melioidosis. The purpose of this study was to examine the role of genomic islands (GIs) as sources of genomic diversity in this species. We found that genomic islands (GIs) vary greatly among B. pseudomallei strains. We identified 71 distinct GIs from the genome sequences of five reference strains of B. pseudomallei: K96243, 1710b, 1106a, MSHR668, and MSHR305. The genomic positions of these GIs are not random, as many of them are associated with tRNA gene loci. In particular, the 3' end sequences of tRNA genes are predicted to be involved in the integration of GIs. We propose the term "tRNA-mediated site-specific recombination" (tRNA-SSR) for this mechanism. In addition, we provide a GI nomenclature that is based upon integration hotspots identified here or previously described. Our data suggest that acquisition of GIs is one of the major sources of genomic diversity within B. pseudomallei and the molecular mechanisms that facilitate horizontally-acquired GIs are common across multiple strains of B. pseudomallei. The differential presence of the 71 GIs across multiple strains demonstrates the importance of these mobile elements for shaping the genetic composition of individual strains and populations within this bacterial species.
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影响因子：
7
作者：
Vernikos, Georgios S.;Parkhill, Julian
通讯作者：
Parkhill, Julian
影响因子：
9.4
作者：
Maharjan, B;Chantratita, N;Peacock, SJ
通讯作者：
Peacock, SJ
DOI：
10.1073/pnas.93.25.14648
发表时间：
1996-12-10
影响因子：
11.1
作者：
Censini, S;Lange, C;Covacci, A
通讯作者：
Covacci, A
影响因子：
9.4
作者：
Korbsrisate, S;Suwanasai, N;Sarasombath, S
通讯作者：
Sarasombath, S
影响因子：
5.8
作者：
Stothard, P;Wishart, DS
通讯作者：
Wishart, DS