Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models.
Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models.
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DOI：
10.3390/life11111267
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发表时间：
2021-11-19
期刊：
影响因子：
--
通讯作者：
Juranek JK
中科院分区：
文献类型：
--
作者：
Jaroslawska J;Korytko A;Zglejc-Waszak K;Antonowski T;Pomianowski AS;Wasowicz K;Wojtkiewicz J;Juranek JK
该研究的目的是比较实验诱导的 1 型或 2 型糖尿病（T1D 或 T2D）对小鼠周围神经功能、结构和生化特性的影响。 8周龄的C57BL/6小鼠被随机分为三组，包括对照组（CTRL，食物喂养）、STZ（注射链脲佐菌素（STZ））组和HFD（高脂饮食（HFD）喂养）组。经过18周的实验治疗后，HFD小鼠体重增加，血脂水平升高，而STZ小鼠出现高血糖。经过 STZ 治疗的小鼠，在长期未治疗糖尿病后，出现了运动和感觉神经传导速度缺陷。此外，相对于对照纤维，糖尿病前期和糖尿病期的轴突数量较少且形状不规则。两个治疗组的动物在坐骨神经中均表现出明显的 nNOS 过度表达和 SOD1 蛋白表达减少，表明这些小鼠的周围神经系统存在氧化亚硝化应激和无效的抗氧化保护。总的来说，STZ 和 HFD 治疗的小鼠表现出相似的周围神经损伤特征，包括坐骨神经的许多形态学和电生理学病理。虽然高血糖是糖尿病神经病变发病机制的一个重要组成部分，但糖尿病的非高血糖效应，包括血脂异常，也可能在这种病症的发展中发挥重要作用。
The objective of the study was to compare the effects of experimentally induced type 1 or type 2 diabetes (T1D or T2D) on the functional, structural and biochemical properties of mouse peripheral nerves. Eight-week-old C57BL/6 mice were randomly assigned into three groups, including the control (CTRL, chow-fed), STZ (streptozotocin (STZ)-injected), and HFD (high-fat diet (HFD)-fed) group. After 18-weeks of experimental treatment, HFD mice had higher body weights and elevated levels of plasma lipids, while STZ mice developed hyperglycemia. STZ-treated mice, after an extended period of untreated diabetes, developed motor and sensory nerve conduction-velocity deficits. Moreover, relative to control fibers, pre- and diabetic axons were lower in number and irregular in shape. Animals from both treatment groups manifested a pronounced overexpression of nNOS and a reduced expression of SOD1 proteins in the sciatic nerve, indicating oxidative–nitrosative stress and ineffective antioxidant protection in the peripheral nervous system of these mice. Collectively, STZ- and HFD-treated mice revealed similar characteristics of peripheral nerve damage, including a number of morphological and electrophysiological pathologies in the sciatic nerve. While hyperglycemia is a large component of diabetic neuropathy pathogenesis, the non-hyperglycemic effects of diabetes, including dyslipidemia, may also be of importance in the development of this condition.
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影响因子：
7.7
作者：
Juranek JK;Geddis MS;Song F;Zhang J;Garcia J;Rosario R;Yan SF;Brannagan TH;Schmidt AM
通讯作者：
Schmidt AM
影响因子：
4
作者：
Bestall SM;Hulse RP;Blackley Z;Swift M;Ved N;Paton K;Beazley-Long N;Bates DO;Donaldson LF
通讯作者：
Donaldson LF
影响因子：
13.6
作者：
Fujita, Hiroki;Fujishima, Hiromi;Takahashi, Takamune
通讯作者：
Takahashi, Takamune
影响因子：
6.6
作者：
D'Adamo, Ebe;Giannini, Cosimo;Mohn, Angelika
通讯作者：
Mohn, Angelika
影响因子：
15.9
作者：
SCHMIDT, AM;YAN, SD;STERN, D
通讯作者：
STERN, D