Ultrastructural and functional fate of recycled vesicles in hippocampal synapses
Ultrastructural and functional fate of recycled vesicles in hippocampal synapses
复制标题
海马突触再生囊泡的超微结构和功能命运
作者：
Stephanie Rey;Catherine Smith;Milena W. Fowler;Freya Crawford;J. Burden;K. Staras
突触小泡的有效回收被认为对于中央终端的持续信息传递至关重要。然而，回收的囊泡对未来传播事件的具体贡献仍不清楚。在这里，我们利用荧光和时间戳电子显微镜来追踪大鼠海马突触中易释放池（RRP）刺激后内吞的囊泡的功能和位置命运。我们表明，大多数囊泡在活性区附近被回收，但随后在簇中占据随机位置，没有对未来使用的优先偏向。这些囊泡非选择性地排队，以肌动蛋白依赖性方式进一步刺激，向释放位点前进。尽管如此，最近在刺激序列中回收的一小部分囊泡仍然靠近活性区域，并在下一个 RRP 中表现出更优先的用途。我们的研究结果揭示了基于纳米级位置和时间规则的囊泡命运的异质性，为未来池构成的起源提供了新的见解。突触小泡在传输后被有效地回收，但它们对未来信号传导的贡献仍不清楚。 Rey 等人发现，只有一小部分囊泡（通常是最近在刺激序列中检索到的囊泡）保留在活性区附近并表现出特权使用。
Efficient recycling of synaptic vesicles is thought to be critical for sustained information transfer at central terminals. However, the specific contribution that retrieved vesicles make to future transmission events remains unclear. Here we exploit fluorescence and time-stamped electron microscopy to track the functional and positional fate of vesicles endocytosed after readily releasable pool (RRP) stimulation in rat hippocampal synapses. We show that most vesicles are recovered near the active zone but subsequently take up random positions in the cluster, without preferential bias for future use. These vesicles non-selectively queue, advancing towards the release site with further stimulation in an actin-dependent manner. Nonetheless, the small subset of vesicles retrieved recently in the stimulus train persist nearer the active zone and exhibit more privileged use in the next RRP. Our findings reveal heterogeneity in vesicle fate based on nanoscale position and timing rules, providing new insights into the origins of future pool constitution. Synaptic vesicles are efficiently retrieved after transmission but the contribution they make to future signalling remains unclear. Rey et al.find that only a subset of vesicles—typically those retrieved recently in the stimulus train—remain near the active zone and exhibit privileged use.
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16.2
作者：
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64.8
作者：
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