Panax ginseng has anti-infective activity against opportunistic pathogen Pseudomonas aeruginosa by inhibiting quorum sensing, a bacterial communication process critical for establishing infection.
Panax ginseng has anti-infective activity against opportunistic pathogen Pseudomonas aeruginosa by inhibiting quorum sensing, a bacterial communication process critical for establishing infection.
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DOI：
10.1016/j.phymed.2010.03.015
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发表时间：
2010-11
期刊：
影响因子：
7.9
通讯作者：
Mathee, K.
中科院分区：
文献类型：
--
作者：
Song, Z.;Kong, K. F.;Wu, H.;Maricic, N.;Ramalingam, B.;Priestap, H.;Schneper, L.;Quirke, J. M. E.;Hoiby, N.;Mathee, K.
由病原体产生的毒力因子在感染过程中起着重要作用，该过程由称为群体感应（quorum sensing，QS）的细胞间通讯机制调节。铜绿假单胞菌是一种重要的人类机会致病菌，可导致免疫系统受损和囊性纤维化患者感染。铜绿假单胞菌的QS系统使用N-酰化高丝氨酸内酯（阿勒）作为信号分子。先前我们已经证明人参治疗允许患有铜绿假单胞菌肺炎的动物有效地清除细菌感染。我们推测，影响感染结果的能力部分是由于人参对铜绿假单胞菌毒力因子的产生有直接影响。研究了人参对海藻酸钠、蛋白酶和阿勒产量的影响。测定人参提取物对原型铜绿假单胞菌PAO 1及其同基因类粘蛋白变体（PAOmucA 22或PDO 300）的生长和群体感应（QS）控制的毒力因子表达的影响。人参对菌体生长无抑制作用，但能促进胞外蛋白的产生，并能促进褐藻胶的产生。然而，人参抑制LasA和LasB的产生，并下调阿勒分子的合成。人参对铜绿假单胞菌的QS系统有负面影响，这可能是我们先前的动物研究中人参在体内帮助细菌从动物肺中清除的机制的一部分。人参的增强和抑制活性可能是相互排斥的，因为它是一种复杂的混合物，如本研究中进行的人参热水提取物的HPLC分析所示。虽然人参是一种很有前途的天然药物，但分离和评价与抗QS活性相关的人参化合物是重要的。
Virulent factors produced by pathogens play an important role in the infectious process, which is regulated by a cell-to-cell communication mechanism called quorum sensing (QS). Pseudomonas aeruginosa is an important opportunistic human pathogen, which causes infections in patients with compromised immune systems and cystic fibrosis. The QS systems of P. aeruginosa use N-acylated homoserine lactone (AHL) as signal molecules. Previously we have demonstrated that Panax ginseng treatment allowed the animals with P. aeruginosa pneumonia to effectively clear the bacterial infection. We postulated that the ability to impact the outcome of infections is partly due to ginseng having direct effect on the production of P. aeruginosa virulence factors. The study explores the effect of ginseng on alginate, protease and AHL production. The effect of ginseng extracts on growth and expression of quorum-sensing (QS)-controlled virulence factors on the prototypic P. aeruginosa PAO1 and its isogenic mucoid variant (PAOmucA22 or PDO300) was determined. Ginseng did not inhibit the growth of the bacteria, enhanced the extracellular protein production and stimulated the production of alginate. However, ginseng suppressed the production of LasA and LasB and down-regulated the synthesis of the AHL molecules. Ginseng has a negative effect on the QS system of P. aeruginosa, which might be part of the mechanisms that ginseng helped the bacterial clearance from the animal lungs in vivo in our previous animal study. It is possible that enhancing and repressing activities of ginseng are mutually exclusive as it is a complex mixture, as shown with the HPLC anaylsis of the hot water extract of ginseng that was performed in this study. Though ginseng is a promising natural synergetic remedy, it is important to isolate and evaluate the ginseng compounds associated with the anti-QS activity.
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DOI：
10.1164/ajrccm.152.4.7551392
发表时间：
1995-10-01
影响因子：
24.7
作者：
JOHANSEN, HK;HOUGEN, HP;HOIBY, N
通讯作者：
HOIBY, N
影响因子：
4.6
作者：
Johansen, HK;Hougen, HP;Hoiby, N
通讯作者：
Hoiby, N
影响因子：
3.1
作者：
Lee, Ji-Hye;Shim, Jin Sun;Kim, Kyung Hyun
通讯作者：
Kim, Kyung Hyun
影响因子：
3.1
作者：
JOHANSEN, HK;ESPERSEN, F;HOIBY, N
通讯作者：
HOIBY, N
影响因子：
2.9
作者：
KNUTSON, CA;JEANES, A
通讯作者：
JEANES, A