Genomic Insights into the Origin and Evolution of Molluscan Red-Bloodedness in the Blood Clam Tegillarca granosa.
Genomic Insights into the Origin and Evolution of Molluscan Red-Bloodedness in the Blood Clam Tegillarca granosa.
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对血蛤 Tegillarca granosa 中软体动物红血性起源和进化的基因组见解。
DOI：
10.1093/molbev/msab030
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发表时间：
2021-05-19
影响因子：
10.7
通讯作者：
Lin Z
中科院分区：
文献类型：
--
作者：
Bao Y;Zeng Q;Wang J;Zhang Z;Zhang Y;Wang S;Wong NK;Yuan W;Huang Y;Zhang W;Liu J;Lv L;Xue Q;Zha S;Peng Z;Yao H;Bao Z;Wang S;Lin Z
血蛤与其软体动物亲属不同，表现出独特的红血（RB）表型;然而，这种进化创新的遗传基础和生化机制仍不清楚。作为解决这个谜团的基本步骤，我们提出了第一个染色体水平的基因组和全面的转录组的泥蚶的综合基因组，进化和功能分析蛤RB表型。我们确定了血蛤特异性和扩展的基因家族，以及RB相关的基因通路。蛤特异性RB相关血红蛋白（Hbs）与血蛤和其他没有RB表型的软体动物的肌红蛋白（Mbs）显示出密切的系统发育关系，表明蛤特异性Hbs可能在进化上源自Mb谱系。值得注意的是，与脊椎动物Hbs相似，血蛤Hbs以基因簇的形式存在。尽管血蛤和脊椎动物的Hb簇的趋同进化，它们的Hb簇可能起源于一个单一的祖先Mb样基因的基因同源性和同线性分析证明。一套完整的酶编码基因血红素合成的血蛤，血淋巴中的显着表达和类似的脊椎动物，表明收敛的RB相关的血红蛋白和血红素功能的脊椎动物和血蛤。RNA干扰实验证实了血红蛋白和血红素合成关键酶在维持蛤类RB表型中的功能作用。本文提出的高质量的基因组组装和全面的转录组为超多样性的软体动物门提供了新的基因组资源，并为无脊椎动物RB的起源和进化提供了深入的见解。
Blood clams differ from their molluscan kins by exhibiting a unique red-blood (RB) phenotype; however, the genetic basis and biochemical machinery subserving this evolutionary innovation remain unclear. As a fundamental step toward resolving this mystery, we presented the first chromosome-level genome and comprehensive transcriptomes of the blood clam Tegillarca granosa for an integrated genomic, evolutionary, and functional analyses of clam RB phenotype. We identified blood clam-specific and expanded gene families, as well as gene pathways that are of RB relevant. Clam-specific RB-related hemoglobins (Hbs) showed close phylogenetic relationships with myoglobins (Mbs) of blood clam and other molluscs without the RB phenotype, indicating that clam-specific Hbs were likely evolutionarily derived from the Mb lineage. Strikingly, similar to vertebrate Hbs, blood clam Hbs were present in a form of gene cluster. Despite the convergent evolution of Hb clusters in blood clam and vertebrates, their Hb clusters may have originated from a single ancestral Mb-like gene as evidenced by gene phylogeny and synteny analysis. A full suite of enzyme-encoding genes for heme synthesis was identified in blood clam, with prominent expression in hemolymph and resembling those in vertebrates, suggesting a convergence of both RB-related Hb and heme functions in vertebrates and blood clam. RNA interference experiments confirmed the functional roles of Hbs and key enzyme of heme synthesis in the maintenance of clam RB phenotype. The high-quality genome assembly and comprehensive transcriptomes presented herein serve new genomic resources for the super-diverse phylum Mollusca, and provide deep insights into the origin and evolution of invertebrate RB.
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影响因子：
6.5
作者：
Anisimova M;Gil M;Dufayard JF;Dessimoz C;Gascuel O
通讯作者：
Gascuel O
影响因子：
4.9
作者：
Bao W;Kojima KK;Kohany O
通讯作者：
Kohany O
影响因子：
10.7
作者：
Hoang DT;Chernomor O;von Haeseler A;Minh BQ;Vinh LS
通讯作者：
Vinh LS
DOI：
10.1073/pnas.0705710104
发表时间：
2007-11-13
影响因子：
11.1
作者：
Benavides, Gloria A.;Squadrito, Giuseppe L.;Kraus, David W.
通讯作者：
Kraus, David W.
影响因子：
7
作者：
Cantarel, Brandi L.;Korf, Ian;Yandell, Mark
通讯作者：
Yandell, Mark