CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling.
CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling.
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DOI：
10.1093/pcp/pcq129
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发表时间：
2011-01
影响因子：
4.9
通讯作者：
Fukuda H
中科院分区：
文献类型：
--
作者：
Kondo Y;Hirakawa Y;Kieber JJ;Fukuda H
细胞间通讯对于组织和器官发育至关重要。在植物中，分泌型 CLAVATA3/胚胎周围区域相关 (CLE) 肽在组织发育（包括血管发育）的各个方面充当细胞间信号分子。然而，人们对 CLE 配体下游血管发育中发挥作用的细胞内信号通路知之甚少。我们发现，包括 CLE10 在内的 CLE 肽优先在根维管系统中表达，可抑制拟南芥根中原木质部维管形成。 GeneChip 分析显示，CLE10 肽特异性抑制两种 A 型拟南芥反应调节剂 (ARR) ARR5 和 ARR6 的表达，其产物充当细胞分裂素信号传导的负调节剂。 arr5 arr6 根表现出有缺陷的原木质部导管形成。这些结果表明CLE10通过抑制A型ARR基因（包括ARR5和ARR6）的表达来抑制原木质部血管形成。这一发现得到了以下发现的支持：在 arr10 arr12（B 型 ARR 基因的双突变体）背景下，CLE10 不会抑制原木质部血管形成。因此，我们的结果揭示了根中原木质部血管形成中 CLE 信号传导和细胞分裂素信号传导之间的串扰。结合细胞分裂素信号传导在茎尖分生组织中 CLV3/WUS 信号传导途径下游发挥作用的迹象，CLE 和细胞分裂素信号传导途径之间的串扰可能是植物发育中的一个共同特征。
Cell–cell communication is critical for tissue and organ development. In plants, secretory CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides function as intercellular signaling molecules in various aspects of tissue development including vascular development. However, little is known about intracellular signaling pathways functioning in vascular development downstream of the CLE ligands. We show that CLE peptides including CLE10, which is preferentially expressed in the root vascular system, inhibit protoxylem vessel formation in Arabidopsis roots. GeneChip analysis displayed that CLE10 peptides repressed specifically the expression of two type-A Arabidopsis Response Regulators (ARRs), ARR5 and ARR6, whose products act as negative regulators of cytokinin signaling. The arr5 arr6 roots exhibited defective protoxylem vessel formation. These results indicate that CLE10 inhibits protoxylem vessel formation by suppressing the expression of type-A ARR genes including ARR5 and ARR6. This was supported by the finding that CLE10 did not suppress protoxylem vessel formation in a background of arr10 arr12, a double mutant of type-B ARR genes. Thus, our results revealed cross-talk between CLE signaling and cytokinin signaling in protoxylem vessel formation in roots. Taken together with the indication that cytokinin signaling functions downstream of the CLV3/WUS signaling pathway in the shoot apical meristem, the cross-talk between CLE and cytokinin signaling pathways may be a common feature in plant development.
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