Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling.
Rice early flowering1, a CKI, phosphorylates DELLA protein SLR1 to negatively regulate gibberellin signalling.
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水稻早花 1 是一种 CKI，磷酸化 DELLA 蛋白 SLR1 以负向调节赤霉素信号传导
DOI：
10.1038/emboj.2010.75
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发表时间：
2010-06-02
期刊：
影响因子：
11.4
通讯作者：
Xue, Hong-Wei
中科院分区：
文献类型：
--
作者：
Dai, Cheng;Xue, Hong-Wei
植物激素赤霉素（giberellin， GA）在植物生长发育的多个方面起着至关重要的作用。为了研究相关的调控机制，我们分离了水稻早开花突变体el1，该突变体缺乏在植物和动物中都起关键作用的酪蛋白激酶I。el1对GA的反应增强，这与外源GA抑制el1表达一致(3)。生化鉴定表明，EL1特异性磷酸化水稻DELLA蛋白SLR1，为SLR1磷酸化提供了直接证据。在野生型植株中，SLR1过表达会导致严重的矮化表型，而EL1缺乏会显著抑制SLR1对GA信号的负面影响，这表明SLR1对GA信号的负面影响需要EL1的功能。进一步的研究表明，SLR1的磷酸化对于维持其活性和稳定性至关重要，SLR1候选磷酸化位点的突变导致GA信号的改变。该研究表明，EL1是GA反应的一个新的关键调控因子，并为研究酪蛋白激酶I在GA信号传导和植物发育中的活性提供了重要线索。
The plant hormone gibberellin (GA) is crucial for multiple aspects of plant growth and development. To study the relevant regulatory mechanisms, we isolated a rice mutant earlier flowering1, el1, which is deficient in a casein kinase I that has critical roles in both plants and animals. el1 had an enhanced GA response, consistent with the suppression of EL1 expression by exogenous GA(3). Biochemical characterization showed that EL1 specifically phosphorylates the rice DELLA protein SLR1, proving a direct evidence for SLR1 phosphorylation. Overexpression of SLR1 in wild-type plants caused a severe dwarf phenotype, which was significantly suppressed by EL1 deficiency, indicating the negative effect of SLR1 on GA signalling requires the EL1 function. Further studies showed that the phosphorylation of SLR1 is important for maintaining its activity and stability, and mutation of the candidate phosphorylation site of SLR1 results in the altered GA signalling. This study shows EL1 a novel and key regulator of the GA response and provided important clues on casein kinase I activities in GA signalling and plant development.
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影响因子：
44.1
作者：
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影响因子：
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