The novel pituitary polypeptide 7B2 is a highly-conserved protein coexpressed with proopiomelanocortin.
The novel pituitary polypeptide 7B2 is a highly-conserved protein coexpressed with proopiomelanocortin.
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新型垂体多肽 7B2 是一种与阿黑皮素原共表达的高度保守蛋白。
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
B. Jenks
中科院分区：
文献类型：
--
作者：
G. Martens;M. Bussemakers;T. Ayoubi;B. Jenks
在两栖类中间脑下垂体的生物合成活性的前体蛋白质proopiomelanocortin（POMC）的生产可以在生理上操纵; POMC合成是高适应黑色背景的动物和低适应白色的动物。为了研究与POMC基因表达相关的基因，我们采用了一种差异杂交技术，包括筛选垂体cDNA文库与探针来自RNA的非活性和生理激活的中间垂体细胞的两栖动物非洲爪蟾。差异杂交爪蟾垂体cDNA克隆编码的新多肽7 B2。这种未知功能的Mr-21，000分泌颗粒相关蛋白在非洲爪蟾和人类之间高度保守（83%的氨基酸序列相似性）。7 B2结构内的保守片段包括N-末端部分，三对碱性氨基酸，它们是蛋白水解酶的潜在识别位点，以及与推定的GTP结合结构域共享相似性的三个区域。爪蟾垂体中7 B2 mRNA的表达量约为POMC mRNA表达量的3%。在中间垂体的POMC和7 B2 mRNA的量是高得多，在黑色适应蟾蜍比白色适应动物。这些生理诱导的POMC和7 B2 mRNA水平的变化没有发现在垂体前叶。我们的结论是POMC和7 B2基因共表达，这些基因的活性的调制是组织特异性的。
In the amphibian intermediate pituitary gland the biosynthetic activity for production of the precursor protein proopiomelanocortin (POMC) can be physiologically manipulated; POMC synthesis is high in animals adapted to a black background and low in white-adapted animals. In order to study genes associated with POMC gene expression we applied a differential hybridization technique involving screening of a pituitary cDNA library with probes derived from RNA of inactive and physiologically activated intermediate pituitary cells of the amphibian Xenopus laevis. A differentially hybridizing Xenopus pituitary cDNA clone encoded the novel polypeptide 7B2. This Mr-21,000 secretory granule-associated protein of unknown function is shown to be highly conserved between Xenopus and human (83% amino acid sequence similarity). Conserved segments within the 7B2 structure included the N-terminal portion, three pairs of basic amino acids which are potential recognition sites for proteolytic enzymes, and three regions sharing similarity with putative GTP-binding domains. Levels of 7B2 mRNA were about 3% of POMC mRNA levels in Xenopus pituitary glands. In the intermediate pituitary the amount of both POMC and 7B2 mRNA was much higher in black-adapted toads than in white-adapted animals. These physiologically-induced changes in POMC and 7B2 mRNA levels were not found in the anterior pituitary. We conclude that the POMC and 7B2 genes are coexpressed and that modulation of the activity of these genes is tissue-specific.
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DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
多蛋白基因表达：神经内分泌肽多样性的产生。
影响因子：
16.6
作者：
Douglass,J;Civelli,O;Herbert,E
通讯作者：
Herbert,E
两栖动物非洲爪蟾中阿片黑皮素原克隆 cDNA 的核苷酸序列。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Martens,GJ;Civelli,O;Herbert,E
通讯作者：
Herbert,E
DOI：
10.1089/dna.1983.2.329
发表时间：
1983-01-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
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作者：
CATHALA, G;SAVOURET, JF;BAXTER, JD
通讯作者：
BAXTER, JD