Antiviral activities of phosphonoformate sodium to pseudorabies herpesvirus infection in vitro
Antiviral activities of phosphonoformate sodium to pseudorabies herpesvirus infection in vitro
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膦甲酸钠体外抗伪狂犬病疱疹病毒感染的活性
DOI：
10.3109/13880209.2010.538416
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发表时间：
2011-05
影响因子：
3.8
通讯作者：
Sui, Xiuwen
中科院分区：
文献类型：
--
作者：
Ren, Xiaofeng;Li, Guangxing;Sui, Xiuwen
背景：膦甲酸钠（PFS）已被用作抗疱疹病毒药物；然而，在兽医环境中使用 PFS 治疗伪狂犬病疱疹病毒 (PrV) 感染的研究尚未广泛报道。目的：本研究旨在分析PFS对PrV诱导的细胞感染和凋亡的抑制作用。材料和方法：当将 PFS 应用于病毒、病毒感染的细胞以及感染前的细胞时，通过噬菌斑测定来确定 PrV 的感染性。进行PCR扩增PrV的DNA聚合酶、gE、gG和gD基因。通过免疫荧光和流式细胞术分析 PrV 诱导的细胞凋亡。结果：PFS 抑制 PrV 的细胞感染。添加药物减少了凋亡细胞的数量。本研究中通过 PCR 检测到的 DNA 聚合酶和其他病毒结构基因的扩增有所减少，因为药物存在下产生的病毒 DNA 拷贝较少。该药对PrV诱导的细胞凋亡有抑制作用。讨论与结论：PFS对PrV的细胞感染有抑制作用，可作为抗PrV药物或与其他抗PrV药物联合使用。在 PFS 存在的情况下，PrV 诱导的细胞凋亡细胞和病毒 DNA 拷贝减少。
Context: Phosphonoformate sodium (PFS) has been used as an anti-herpesvirus drug; nevertheless, studies of the use of PFS for treatment of pseudorabies herpesvirus (PrV) infection in the veterinary setting have not been widely reported. Objective: The present study aimed to analyze the inhibitory effect of PFS on cell infection and apoptosis induced by PrV. Materials and methods: The infectivity of PrV was determined by plaque assays when PFS was applied to the virus, to the virus-infected cells, and to the cells prior to infection. PCR amplifying DNA polymerase, gE, gG, and gD genes of PrV was performed. PrV-induced cell apoptosis was analyzed by immunofluorescence and flow cytometry. Results: PFS inhibits cell infection by PrV. Addition of the drug decreased the number of apoptotic cells. Amplification of DNA polymerase and other viral structural genes detected in this study by PCR was reduced, because there were fewer viral DNA copies being made in the presence of the drug. The drug has an inhibitory effect on cell apoptosis induced by PrV. Discussion and conclusion: PFS has inhibitory effects on cell infection by PrV, which may be used as an anti-PrV agent or combined with other anti-PrV agents. PrV-induced cell apoptotic cells and viral DNA copies decreased in the presence of the PFS.
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影响因子：
15.9
作者：
Kedes, DH;Ganem, D
通讯作者：
Ganem, D
影响因子：
5.4
作者：
B. Klupp;C. Hengartner;T. Mettenleiter;L. Enquist
通讯作者：
B. Klupp;C. Hengartner;T. Mettenleiter;L. Enquist
影响因子：
2.7
作者：
Davison, Andrew J.;Eberle, Richard;Ehlers, Bernhard;Hayward, Gary S.;McGeoch, Duncan J.;Minson, Anthony C.;Pellett, Philip E.;Roizman, Bernard;Studdert, Michael J.;Thiry, Etienne
通讯作者：
Thiry, Etienne
伪狂犬病疱疹病毒进入的胆固醇依赖性
影响因子：
2.6
作者：
Ren X;Yin J;Li G;Herrler G
通讯作者：
Herrler G
甘草酸二铵和氯化锂对伪狂犬病疱疹病毒细胞感染的抗病毒作用
影响因子：
7.6
作者：
Sui X;Yin J;Ren X
通讯作者：
Ren X