Remifentanil Preconditioning Attenuates Hepatic Ischemia-Reperfusion Injury in Rats via Neuronal Activation in Dorsal Vagal Complex.
Remifentanil Preconditioning Attenuates Hepatic Ischemia-Reperfusion Injury in Rats via Neuronal Activation in Dorsal Vagal Complex.
复制标题
瑞芬太尼预处理通过背侧迷走神经复合体神经元激活减轻大鼠肝脏缺血再灌注损伤
DOI：
10.1155/2018/3260256
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发表时间：
2018
影响因子：
4.6
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Cui C;Yu F;Yin S;Yang Y;Jiao Y;Cheung C;Wang X;Qi B;Liu Y;Li P;Yu W;Xiao J;Yang L
瑞芬太尼是一种超短效阿片类药物，有报道称其可减轻肝脏缺血 - 再灌注损伤，而肝脏缺血 - 再灌注损伤是术后肝功能障碍的主要原因。本研究旨在确定中枢迷走神经通路是否参与这一保护过程。实验过程中使用了大鼠肝脏缺血 - 再灌注模型。结果显示，静脉注射瑞芬太尼预处理可降低血清转氨酶水平并减轻肝脏组织学损伤；然而，腹腔注射μ - 阿片受体拮抗剂并不能消除瑞芬太尼预处理的保护作用。脑干的c - Fos免疫荧光检测表明，瑞芬太尼预处理后迷走神经背核被激活。此外，与接受瑞芬太尼预处理的迷走神经切断动物相比，瑞芬太尼预处理组的血清丙氨酸转氨酶水平、组织病理学损伤及细胞凋亡均有所降低，且生理盐水/迷走神经切断组（NS/Va）与瑞芬太尼预处理/迷走神经切断组（RPC/Va）之间的肿瘤坏死因子 - α（TNF - α）和白细胞介素 - 6（IL - 6）水平无统计学差异。另外，向迷走神经背侧复合体微量注射瑞芬太尼可降低血清转氨酶、炎性细胞因子水平，减轻肝脏组织学损伤及细胞凋亡，而这些作用也会被外周肝迷走神经切断所消除。总之，瑞芬太尼预处理对肝脏缺血 - 再灌注损伤具有保护作用，且这一作用由中枢迷走神经通路介导。
Remifentanil, an ultra-short acting opiate, has been reported to protect against hepatic ischemia-reperfusion injury, which is a major cause of postoperative liver dysfunction. The objective of this study was to determine whether a central vagal pathway is involved in this protective procedure. Rat models of hepatic ischemia-reperfusion were used in the experimental procedures. The results revealed that intravenous pretreatment with remifentanil decreased serum aminotransferases and hepatic histologic damage; however, an intraperitoneal injection of μ-opioid receptor antagonist did not abolish the protection of remifentanil preconditioning. c-Fos immunofluorescence of the brain stem showed that dorsal motor nucleus of the vagus was activated after remifentanil preconditioning. Moreover, serum alanine aminotransferase, histopathologic damage, and apoptosis decreased in remifentanil preconditioning group compared to vagotomized animals with remifentanil preconditioning, and there was no statistical difference of TNF-α and IL-6 between NS/Va and RPC/Va groups. In addition, remifentanil microinjection into dorsal vagal complex decreased serum aminotransferases, inflammatory cytokines, and hepatic histologic injury and apoptosis, and these effects were also abolished by a peripheral hepatic vagotomy. In conclusion, remifentanil preconditioning conferred liver protection against ischemia-reperfusion injury, which was mediated by the central vagal pathway.
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