Complete genome sequence of a novel capillovirus infecting Hevea brasiliensis in China

Complete genome sequence of a novel capillovirus infecting Hevea brasiliensis in China
复制标题

感染中国橡胶树的新型毛状病毒的全基因组序列

DOI:
10.1007/s00705-019-04459-8
复制
发表时间:
2019-11
影响因子:
2.7
通讯作者:
Xi Huang
Xi Huang
中科院分区:
医学4区
文献类型:
--
作者:
Zhaotong Li;Hongxing Wang;Ruibai Zhao;Ze Zhang;Zhihui Xia;Jinling Zhai;Xi Huang

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割胶面板干燥(TPD)是一种复杂的疾病,在橡胶树(橡胶树)割胶时会导致胶乳部分或完全停止排水。在这项工作中,我们测定了一种新病毒的全基因组序列,该病毒是在中国的一棵橡胶树上发现的,患有TPD综合征。该病毒基因组全长6811个核苷酸,具有两个重叠的开放阅读框(ORF1和ORF2),分别编码一个多蛋白和一个运动蛋白。该蛋白与樱桃病毒A(CVA,ARQ83874.1)的氨基酸序列同源性为37%,覆盖率超过99%。基因组结构类似于毛细病毒属(Betafexiviridae)的成员。对复制酶蛋白的系统发育分析表明,该病毒与毛细管病毒属的成员聚在一起。新病毒暂定名为“橡胶树病毒1”(RTV1)。RTV1是第一个被报道感染橡胶树的病毒。本工作为寻找巴西橡胶树TPD的潜在致病因子奠定了基础
Tapping panel dryness (TPD) is a complex disorder that causes partial or complete cessation of latex drainage upon tapping of rubber trees (Hevea brasiliensis). In this work, we determined the complete genome sequences of a novel virus identifed in a rubber tree with TPD syndrome in China. The genome of the virus consists of 6811 nt and possesses two overlapping open reading frames (ORF1 and ORF2), encoding a polyprotein and a movement protein, respectively. The polyprotein shares 37% amino acid sequence identity with cherry virus A (CVA, ARQ83874.1) over 99% coverage. The genome architecture is similar to that of members of the genus Capillovirus (family Betafexiviridae). Phylogenetic analysis of the replicase proteins showed that the virus clustered together with members of the genus Capillovirus. The new virus is tentatively called “rubber tree virus 1” (RTV1). RTV1 is the frst virus reported to infect rubber trees. This work lays a foundation for research into fnding the potential causal agent of TPD in Hevea brasiliensis
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