Antioxidant effect of a phytoestrogen equol on cultured muscle cells of embryonic broilers
Antioxidant effect of a phytoestrogen equol on cultured muscle cells of embryonic broilers
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植物雌激素雌马酚对肉鸡培养肌细胞的抗氧化作用
DOI：
10.1007/s11626-011-9464-x
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发表时间：
2011-11
影响因子：
2.1
通讯作者：
Wei, Xiao-Jing
中科院分区：
文献类型：
--
作者：
Lu, Li-Zhi;Wu, Jing;Zhao, Ru-Qian;Ni, Ying-Dong;Wei, Xiao-Jing
已有研究表明，卵内注射雌马酚可通过提高抗氧化状态，显著提高7周龄肉仔鸡肌肉的持水性能。本研究旨在直接研究雌马酚对肉仔鸡肌肉细胞的抗氧化作用。肌细胞从胚胎的腿肌分离的第11天的孵化和处理的雌马酚和H2 O2，单独或一起。用含1、10或100 μM雌马酚的培养基预处理细胞1 h，然后再加入1 mM H2 O2 1 h。获得细胞的显微照片。测定细胞存活率、细胞上清液中丙二醛（MDA）含量、L-乳酸脱氢酶（LDH）活性以及细胞内总超氧化物歧化酶（T-SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。用1 mM H2 O2处理对细胞造成严重损伤，表现为彗星没有清晰的头部区域，但有非常明显的DNA片段尾部。低浓度（1 μM）的雌马酚预处理对细胞损伤有抑制作用，而高浓度（10 μM）的雌马酚预处理对细胞损伤有抑制作用，而100 μM的雌马酚预处理对细胞的损伤比单独使用H2 O2更严重。用1 μM雌马酚预处理对细胞活力没有影响，而用10和100 μM雌马酚预处理以剂量依赖性方式显著降低细胞活力。与单纯H2 O2相比，低剂量雌马酚预处理可显著降低细胞上清液中LDH活性和MDA含量，显著提高细胞内T-SOD活性（P< 0.05），并有提高细胞内GSH-Px活性的趋势（0.05 <P< 0.1）。高剂量雌马酚（10 μM和100 μM）预处理可显著提高LDH活性，但对H_2O_2诱导的MDA含量、T-SOD和GSH-Px活性无明显影响，但对GSH-Px活性有显著影响。结果表明，低剂量雌马酚可预防H2 O2诱导的骨骼肌细胞损伤，而高剂量雌马酚预处理与H2 O2诱导的细胞损伤具有协同作用。
Previous studies have shown that the in ovo injection of equol can markedly improve the water-holding capacity of muscles of broilers chickens at 7 wk of age through promotion of the antioxidant status. We aimed to investigate directly the antioxidant effects of equol on muscle cells in broilers. Muscle cells were separated from leg muscle of embryos on the 11th day of incubation and treated with equol and H2O2, either alone or together. Cells were pretreated with medium containing 1, 10, or 100 μM equol for 1 h prior to the addition of 1 mM H2O2for a further 1 h. Photomicrographs of cells were obtained. Cell viability, malondialdehyde (MDA) content, and L-lactate dehydrogenase (LDH) activity in the cell supernatant, as well as intracellular total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) activities were determined. Treatment with 1 mM H2O2caused serious damage to cells, indicated by comets with no clear head region but a very apparent tail of DNA fragments. Pretreatment with low (1 μM) but not high concentrations of equol (10 μM) inhibited cell damage, while 100 μM equol caused more serious damage than H2O2alone. Pretreatment with 1 μM equol had no effect on cell viability, while pretreatment with 10 and 100 μM equol significantly decreased cell viability in a dose-dependent manner. Compared with H2O2alone, pretreatment with low-dosage equol markedly decreased LDH activity and MDA production in the supernatant, significantly increased intracellular T-SOD activity (P< 0.05) and tended to increase intracellular GSH-Px activity (0.05 <P< 0.1). Pretreatment with high-dosage equol (10 and 100 μM) significantly enhanced LDH activity, but had no effect on MDA content, T-SOD or GSH-Px activity induced by H2O2,except for an obvious increase in GSH-Px activity caused by 10 μM equol. These results indicate that equol at low dosage can prevent skeletal muscle cell damage induced by H2O2, while pretreatment with high-dosage equol shows a synergistic effect with H2O2in inducing cell damage.
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影响因子：
4.1
作者：
AXELSON, M;KIRK, DN;SETCHELL, KDR
通讯作者：
SETCHELL, KDR
影响因子：
168.9
作者：
CERUTTI, PA
通讯作者：
CERUTTI, PA
DOI：
10.1016/j.bbrc.2008.08.027
发表时间：
2008-10-24
影响因子：
3.1
作者：
Chung, Jae-Eun;Kim, Sue Yeon;Kim, Mee-Ran
通讯作者：
Kim, Mee-Ran
DOI：
10.1073/pnas.90.7.2690
发表时间：
1993-04-01
影响因子：
11.1
作者：
FOTSIS, T;PEPPER, M;SCHWEIGERER, L
通讯作者：
SCHWEIGERER, L
影响因子：
3.5
作者：
Muthyala, RS;Ju, YH;Katzenellenbogen, JA
通讯作者：
Katzenellenbogen, JA