Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection.
Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection.
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DOI：
10.1186/1471-2164-9-325
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发表时间：
2008-07-09
期刊：
影响因子：
4.4
通讯作者：
Wang A
中科院分区：
文献类型：
--
作者：
Babu M;Griffiths JS;Huang TS;Wang A
病毒感染诱导宿主体内全局基因表达的激活和抑制。分析宿主基因表达的变化可能为宿主对病毒感染的生理和表型反应的分子机制提供见解。本研究利用拟南芥Affymetrix ATH1基因阵列分析了拟南芥感染梅痘病毒（Plum pox virus， PPV）后基因表达的变化。为了鉴定响应PPV感染的早期基因，建立了拟南芥同步单细胞转化系统。用PPV感染克隆转染拟南芥原生质体，分析了转染原生质体中基因表达的变化。对ppv感染的拟南芥叶片组织进行微阵列分析，发现2013个基因显著（Q≤0.05）上调（≥2.5倍），1457个基因显著（Q≤0.05）下调（≤-2.5倍）。与可溶性糖、淀粉和氨基酸、细胞膜/膜结合细胞器、叶绿体和蛋白质命运相关的基因上调，而与发育/储存蛋白、蛋白质合成和翻译以及细胞壁相关成分相关的基因下调。这些基因表达变化与PPV感染和症状发展有关。用PPV感染克隆转染原生质体的进一步转录谱分析显示，早在转染后6小时，防御和细胞信号基因就上调了。通过对PPV侵染拟南芥叶片差异调控基因与PPV天然寄主桃李（Prunus persica）叶片uniEST序列的交叉比对分析，鉴定出与防御、代谢和蛋白质合成相关的同源基因。交叉比较PPV感染与其他阳性意义RNA病毒感染差异调控的基因，发现共有416个基因。这些已确定的基因，特别是早期反应基因，可能在病毒感染中起关键作用。PPV感染的拟南芥基因表达变化是胁迫和防御样反应、PPV发病机制和症状发展的分子基础。本研究中发现的差异调控基因，特别是早期应答基因，以及受PPV和其他阳性RNA病毒感染调控的一组共同基因，适合进一步进行功能表征，以阐明分子病毒与宿主的相互作用。
Virus infection induces the activation and suppression of global gene expression in the host. Profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. In this study, the Arabidopsis Affymetrix ATH1 array was used to assess global gene expression changes in Arabidopsis thaliana plants infected with Plum pox virus (PPV). To identify early genes in response to PPV infection, an Arabidopsis synchronized single-cell transformation system was developed. Arabidopsis protoplasts were transfected with a PPV infectious clone and global gene expression changes in the transfected protoplasts were profiled. Microarray analysis of PPV-infected Arabidopsis leaf tissues identified 2013 and 1457 genes that were significantly (Q ≤ 0.05) up- (≥ 2.5 fold) and downregulated (≤ -2.5 fold), respectively. Genes associated with soluble sugar, starch and amino acid, intracellular membrane/membrane-bound organelles, chloroplast, and protein fate were upregulated, while genes related to development/storage proteins, protein synthesis and translation, and cell wall-associated components were downregulated. These gene expression changes were associated with PPV infection and symptom development. Further transcriptional profiling of protoplasts transfected with a PPV infectious clone revealed the upregulation of defence and cellular signalling genes as early as 6 hours post transfection. A cross sequence comparison analysis of genes differentially regulated by PPV-infected Arabidopsis leaves against uniEST sequences derived from PPV-infected leaves of Prunus persica, a natural host of PPV, identified orthologs related to defence, metabolism and protein synthesis. The cross comparison of genes differentially regulated by PPV infection and by the infections of other positive sense RNA viruses revealed a common set of 416 genes. These identified genes, particularly the early responsive genes, may be critical in virus infection. Gene expression changes in PPV-infected Arabidopsis are the molecular basis of stress and defence-like responses, PPV pathogenesis and symptom development. The differentially regulated genes, particularly the early responsive genes, and a common set of genes regulated by infections of PPV and other positive sense RNA viruses identified in this study are candidates suitable for further functional characterization to shed lights on molecular virus-host interactions.
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DOI：
10.1007/1-4020-3780-5_14
发表时间：
2006-01-01
期刊：
NATURAL RESISTANCE MECHANISMS OF PLANTS TO VIRUSES
影响因子：
--
作者：
Handford, Michael G.;Carr, John P.
通讯作者：
Carr, John P.
影响因子：
5.8
作者：
Berriz, GF;King, OD;Roth, FP
通讯作者：
Roth, FP
影响因子：
4.9
作者：
Herbers, K.;Takahata, Y.;Sonnewald, U.
通讯作者：
Sonnewald, U.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
6.9
作者：
Diaz-Vivancos, P.;Rubio, M.;Hernandez, J. A.
通讯作者：
Hernandez, J. A.