JAK2 and PTPRC mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
JAK2 and PTPRC mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
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系统性红斑狼疮患者外周血单个核细胞中JAK2和PTPRC mRNA的表达
DOI：
10.1007/s10067-019-04778-w
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发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
Xuejun Zhang
中科院分区：
文献类型：
--
作者：
Danfeng Qian;Lu Liu;Tingting Zhu;Leilei Wen;Zhengwei Zhu;Xianyong Yin;Ying Qiu;Mingshun Wu;Xueying Li;Jie Ma;Qun Zhang;Ling Jin;Xiaomeng Wang;Qiaohu Xu;Sen Yang;Yujun Sheng;Sheng-Quan Zhang;Xuejun Zhang
本研究旨在探讨SLE患者和对照者外周血单核细胞（PBMC）中JAK2和PTPRC的表达水平，检测SLE活动对基因mRNA表达的影响，并寻找基因mRNA表达与患者临床表现之间的关联。我们进行了定量实时 PCR (qRT-PCR)，以测试从 135 名 SLE 患者和 130 名健康对照中提取的 PBMC 中 JAK2 和 PTPRC 表达水平的差异。此外，我们通过表达数量性状位点（eQTL）检测了SNP对基因表达的调节作用。我们还测试了基因 mRNA 表达是否受到 SLE 活动的影响，并分析了基因 mRNA 表达与患者临床表现之间的关系。 SLE患者JAK2 mRNA表达水平显着高于健康对照者（P=0.005），PTPRC mRNA表达水平显着降低（P<0.001）。然而，没有检测到其他统计显着性。我们发现JAK2 mRNA表达升高和PTPRC mRNA表达降低可能在SLE发病中发挥作用。要点• SLE患者中JAK2 mRNA表达水平显着高于健康对照者。• SLE中PTPRC mRNA表达水平降低。• JAK2和PTPRC mRNA表达可能在SLE发病中发挥提示作用。
In this study, we aimed to explore the expression levels of JAK2 and PTPRC in peripheral blood mononuclear cells (PBMCs) from SLE patients and controls, detect the effects of SLE activity on genes mRNA expression, and find the association between genes mRNA expression and clinical manifestations of patients. We performed quantitative real-time PCR (qRT-PCR) to test differences in the expression levels of JAK2 and PTPRC in PBMCs extracted from 135 patients with SLE and 130 healthy controls. Furthermore, we detected the regulatory effect of SNPs on gene expression by expression quantitative trait loci (eQTL). We also tested whether the genes mRNA expression was affected with the SLE activity and analyzed the relationship between genes mRNA expression and clinical manifestations of patients. The mRNA expression levels of JAK2 in SLE patients were significantly higher than those in healthy controls (P = 0.005), and PTPRC mRNA expression levels were significantly decreased (P < 0.001). However, no other statistical significance was detected. We found that the elevated JAK2 mRNA expression and the decreased PTPRC mRNA expression may play suggestive roles in the pathogenesis of SLE.Key Points• The JAK2 mRNA expression levels in SLE patients were significantly higher than those in healthy controls.• The PTPRC mRNA expression levels in SLE were decreased.• JAK2 and PTPRC mRNA expression may play suggestive roles in the pathogenesis of SLE.
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中国和欧洲个体的全基因组关联荟萃分析确定了与系统性红斑狼疮相关的十个新位点
影响因子：
30.8
作者：
Morris DL;Sheng Y;Zhang Y;Wang YF;Zhu Z;Tombleson P;Chen L;Cunninghame Graham DS;Bentham J;Roberts AL;Chen R;Zuo X;Wang T;Wen L;Yang C;Liu L;Yang L;Li F;Huang Y;Yin X;Yang S;Rönnblom L;Fürnrohr BG;Voll RE;Schett G;Costedoat-Chalumeau N;Gaffney PM;Lau YL;Zhang X;Yang W;Cui Y;Vyse TJ
通讯作者：
Vyse TJ
DOI：
10.24875/gmm.m19000234
发表时间：
2019-05
期刊：
Gaceta de M�xico
影响因子：
--
作者：
E. Layseca-Espinosa;A. Monsiváis-Urenda;L. Doníz-Padilla;H. Portillo-Salazar;B. Hernández-Castro;M. Vitales-Noyola;R. González-Amaro
通讯作者：
E. Layseca-Espinosa;A. Monsiváis-Urenda;L. Doníz-Padilla;H. Portillo-Salazar;B. Hernández-Castro;M. Vitales-Noyola;R. González-Amaro
影响因子：
64.5
作者：
Parganas, E;Wang, D;Ihle, JN
通讯作者：
Ihle, JN
影响因子：
3.5
作者：
R. Duhé;Hallgeir Rui;John D. Greenwood;Kevin J. Garvey;William L. Farrar
通讯作者：
R. Duhé;Hallgeir Rui;John D. Greenwood;Kevin J. Garvey;William L. Farrar
影响因子：
4.1
作者：
Cornez, Isabelle;Yajnanarayana, Sowmya Parampalli;Wolf, Dominik
通讯作者：
Wolf, Dominik