Isolation of Antagonistic Endophytes from Banana Roots against Meloidogyne javanica and Their Effects on Soil Nematode Community.
Isolation of Antagonistic Endophytes from Banana Roots against Meloidogyne javanica and Their Effects on Soil Nematode Community.
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香蕉根部爪哇根结线虫拮抗内生菌的分离及其对土壤线虫群落的影响
DOI：
10.3389/fmicb.2017.02070
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发表时间：
2017
影响因子：
5.2
通讯作者：
Shen Q
中科院分区：
文献类型：
--
作者：
Su L;Shen Z;Ruan Y;Tao C;Chao Y;Li R;Shen Q
香蕉生产受到根结线虫属的严重阻碍。世界各地。与农药相比，内生菌是一种理想的环境友好剂。在本研究中，从感染根结线虫属的香蕉根中分离出内生菌。在体外以及在无菌和天然香蕉单一栽培土壤中对具有不同疾病水平的爪哇根结线虫进行了测试。中、高病害根部拮抗内生菌比例较高。其中，以细菌为主，假单胞菌属、芽孢杆菌属等。和链霉菌属。显示出更丰富的种群数量。分离得到一株具有一定根内定殖能力的菌株，命名为SA，并鉴定为链霉菌属(Streptomyces sp.)。与对照相比，该菌株对爪哇根结线虫的体外抑制率>50%，在无菌土壤中的生防效率为70.7%。温室试验结果表明，SA菌株对根部和根结线虫侵染土壤中的植物寄生虫均表现出优异的生物防治能力。 SA处理显示食菌动物数量较多，特别是中杆线虫和头叶线虫。 SA处理中成熟度指数显着降低，而富集指数（EI）显着升高。总之，本研究展示了内生菌株链霉菌的重要潜在应用。用于控制植物寄生线虫，特别是爪哇根结线虫，并介绍了对线虫群落相关变异的影响。
Banana production is seriously hindered by Meloidogyne spp. all over the world. Endophytes are ideal candidates compared to pesticides as an environmentally benign agent. In the present study, endophytes isolated from banana roots infected by Meloidogyne spp. with different disease levels were tested in vitro, and in sterile and nature banana monoculture soils against Meloidogyne javanica. The proportion of antagonistic endophytes were higher in the roots of middle and high disease levels. Among those, bacteria were dominant, and Pseudomonas spp., Bacillus spp. and Streptomyces spp. showed more abundant populations. One strain, named as SA, with definite root inner-colonization ability was isolated and identified as Streptomyces sp. This strain showed an inhibiting rate of >50% in vitro and biocontrol efficiency of 70.7% in sterile soil against Meloidogyne javanica, compared to the control. Greenhouse experiment results showed that the strain SA exhibits excellent biological control ability for plant-parasites both in roots and in root-knot nematode infested soil. SA treatment showed a higher number of bacterivores, especially Mesorhabditis and Cephalobus. The maturity index was significantly lower, while enrichment index (EI) was significantly higher in the SA treatment. In conclusion, this study presents an important potential application of the endophytic strain Streptomyces sp. for the control of plant-parasitic nematodes, especially Meloidogyne javanica, and presents the effects on the associated variation of the nematode community.
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DOI：
10.1023/b:wibi.0000040406.30495.48
发表时间：
2004-07-01
影响因子：
4.1
作者：
Cao, LX;Qiu, ZQ;Zhou, SN
通讯作者：
Zhou, SN
影响因子：
4.8
作者：
DuPont, S. Tianna;Ferris, Howard;Van Horn, Mark
通讯作者：
Van Horn, Mark
影响因子：
3.3
作者：
Igarashi, Y;Iida, T;Furumai, T
通讯作者：
Furumai, T
影响因子：
4.1
作者：
Li, Rong;Li, Linzhi;Shen, Qirong
通讯作者：
Shen, Qirong
影响因子：
2.1
作者：
Cao, LX;Qiu, ZQ;Zhou, SN
通讯作者：
Zhou, SN