NF-κB subunits are differentially distributed in cells of lumbar dorsal root ganglia in naïve and diabetic rats.
NF-κB subunits are differentially distributed in cells of lumbar dorsal root ganglia in naïve and diabetic rats.
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NF-κB 亚基在幼稚大鼠和糖尿病大鼠的腰背根神经节细胞中分布存在差异。
DOI：
10.1016/j.neulet.2010.12.022
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发表时间：
2011-02-18
影响因子：
2.5
通讯作者：
Siegel RE
中科院分区：
文献类型：
--
作者：
Berti-Mattera LN;Larkin B;Hourmouzis Z;Kern TS;Siegel RE
既往研究表明，链脲佐菌素（STZ）诱导的糖尿病大鼠背根神经节（DRG）核因子κB （NF-κB）活化降低。DRG包含神经元的细胞体，传递来自外周的感觉信号。为了确定糖尿病神经病变与DRG中NF-κB表达的关系，我们对naïve和3月龄糖尿病大鼠进行了行为学、免疫组织化学和生化研究。行为学研究证实，许多患有糖尿病的大鼠后爪会出现触觉异常，或对轻触的敏感性增加。免疫组化研究显示，p50和p65是NF-κB常见的亚基伙伴，它们的定位存在差异。小、中型神经元细胞质及卫星细胞均可见强烈的p65免疫染色。相比之下，p50几乎定位于所有神经元的细胞质中。在许多病例中，细胞核也有明显的染色，这与转录因子激活的位置一致。免疫组织化学和生化研究发现，与naïve动物相比，糖尿病大鼠p50表达的核质比明显降低。我们的研究结果提出了DRG神经元亚群中NF-κB活化变化参与介导糖尿病诱导的感觉神经病变的可能性。
Previous studies demonstrated that Nuclear Factor κB (NF-κB) activation is decreased in dorsal root ganglia (DRG) of rats having streptozotocin (STZ) - induced diabetes. DRG contain cell bodies of neurons that convey sensory signals from the periphery. To determine the relationship between diabetes-induced neuropathy and NF-κB expression in DRG, behavioral, immunohistochemical, and biochemical studies were performed on naïve and 3-month diabetic rats. Behavioral studies confirmed that many diabetic rats develop tactile allodynia, or increased sensitivity to light touch, in the hind paws. Immunohistochemical studies on lumbar DRG that receive input from the affected regions revealed that p50 and p65, frequent NF-κB subunit partners, are differentially localized. Intense p65 immunostaining was detected in the cytoplasm of small- and medium-sized neurons as well as in satellite cells. In contrast, p50 was localized in the cytoplasm of virtually all neurons. In many cases, prominent staining was also present in nuclei, a location consistent with transcription factor activation. Immunohistochemical and biochemical studies found that the nuclear to cytoplasmic ratio of p50 expression was significantly reduced in diabetic rats compared to that in naïve animals. Our findings raise the possibility that changes in NF-κB activation in a subset of DRG neurons participates in mediating diabetes-induced sensory neuropathy.
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影响因子：
7.4
作者：
Fu ES;Zhang YP;Sagen J;Candiotti KA;Morton PD;Liebl DJ;Bethea JR;Brambilla R
通讯作者：
Brambilla R
DOI：
10.1016/j.biocel.2007.05.004
发表时间：
2008-01-01
影响因子：
4
作者：
Pereira, Silvia Gaspar;Oakley, Fiona
通讯作者：
Oakley, Fiona
影响因子：
15.1
作者：
Dublin, Pavel;Hanani, Menachem
通讯作者：
Hanani, Menachem
影响因子：
3.5
作者：
Hamanoue, M;Middleton, G;Davies, AM
通讯作者：
Davies, AM
影响因子：
5.3
作者：
KALTSCHMIDT, C;KALTSCHMIDT, B;BAEUERLE, PA
通讯作者：
BAEUERLE, PA