Quantitation of human immunodeficiency virus by culture and polymerase chain reaction in response to didanosine after long-term therapy with zidovudine.
Quantitation of human immunodeficiency virus by culture and polymerase chain reaction in response to didanosine after long-term therapy with zidovudine.
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通过培养和聚合酶链反应对齐多夫定长期治疗后对去羟肌苷的反应进行人类免疫缺陷病毒定量。
DOI：
10.1093/infdis/169.2.416
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Merigan,TC
中科院分区：
文献类型：
--
作者：
Katzenstein,DA;Winters,M;Bubp,J;Israelski,D;Winger,E;Merigan,TC
通过定量 RNA 和 DNA 聚合酶链反应 (PCR)、细胞和血浆感染性稀释培养物以及免疫复合物解离 p24 抗原捕获 ELISA，对 10 名接受长期齐多夫定治疗的受试者在治疗改为去羟肌苷前后重复进行人类免疫缺陷病毒的测量。基线测定的比较表明，所有受试者均可测量定量细胞培养物、血浆 RNA 和前病毒 DNA，并且细胞培养结果与核酸测量值显着相关。在 8 名受试者中的 3 名和 10 名受试者中的 6 名受试者的 3 次测量中分别检测到血浆病毒血症（如培养物所示）和 p24 抗原。在开始去羟肌苷治疗后长达 6 个月内，血浆 RNA 和细胞稀释培养物较基线显着降低。这些发现表明，使用去羟肌苷可降低病毒负荷；然而，血浆RNA的持续检测表明需要额外的抗病毒治疗来抑制病毒复制。
Measurements of human immunodeficiency virus by quantitative RNA and DNA polymerase chain reaction (PCR), cell and plasma infectivity dilution cultures, and immune complex-disassociated p24 antigen-capture ELISA were made repeatedly in 10 subjects receiving long-term zidovudine treatment before and after therapy was changed to didanosine. Comparison of baseline assays showed that quantitative cell cultures, plasma RNA, and proviral DNA were measurable in all subjects and that cell culture results were significantly correlated with measures of nucleic acids. Plasma viremia (as indicated by culture) and p24 antigen were detected in three measurements in 3 of 8 and 6 of 10 subjects, respectively. Significant decreases in plasma RNA and cell dilution cultures from baseline were maintained for up to 6 months after initiation of didanosine therapy. These findings demonstrate a decrease in virus burden with the use of didanosine; however, continued detection of plasma RNA suggests that additional antiviral therapy will be required to suppress viral replication.
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DOI：
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PIATAK, M;SAAG, MS;LIFSON, JD
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KAHN, JO;LAGAKOS, SW;DOLIN, R
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DOI：
--
发表时间：
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期刊：
Journal of acquired immune deficiency syndromes
影响因子：
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作者：
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通讯作者：
Merigan,TC