Dynamical localization of a thylakoid membrane binding protein is required for acquisition of photosynthetic competency.
Dynamical localization of a thylakoid membrane binding protein is required for acquisition of photosynthetic competency.
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DOI：
10.1111/mmi.13912
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发表时间：
2018-04
影响因子：
3.6
通讯作者：
O'Shea EK
中科院分区：
文献类型：
--
作者：
Gutu A;Chang F;O'Shea EK
Vipp1是高度保守的，对光合作用至关重要，但其功能尚不清楚，因为它不直接参与光依赖性反应。我们分析了Vipp1在活体蓝藻细胞中的定位，并表明Vipp1是高度动态的，在均匀分布于整个细胞的扩散部分和集中在位于细胞周边的类囊体的高曲率区域的点状部分之间连续交换。通过将Vipp1重新定位到类核来实验性地扰动Vipp1的空间分布，在从非光合作用（暗）到光合作用（光）生长的过渡期间导致严重的生长缺陷。然而，同样的扰动Vipp1在黑暗中单独或光单独的生长条件下导致没有生长或类囊体形态缺陷。我们建议，Vipp1在高类囊体曲率的区域中的细胞周边的间断动力学，使收购光合能力，可能是通过促进光合作用的光依赖性反应中涉及的光合复合物的生物合成。在活的和生长的蓝藻细胞，胞质Vipp1不断集中和关闭在高类囊体曲率的区域。这些间断的动态Vipp1代表类囊体膜上的Vipp1寡聚化的事件，这是必不可少的打开光合代谢，也许是通过介导的功能组装的光合机械。
Vipp1 is highly conserved and essential for photosynthesis, but its function is unclear as it does not participate directly in light-dependent reactions. We analyzed Vipp1 localization in live cyanobacterial cells and show that Vipp1 is highly dynamic, continuously exchanging between a diffuse fraction that is uniformly distributed throughout the cell and a punctate fraction that is concentrated at high curvature regions of the thylakoid located at the cell periphery. Experimentally perturbing the spatial distribution of Vipp1 by relocalizing it to the nucleoid causes a severe growth defect during the transition from non-photosynthetic (dark) to photosynthetic (light) growth. However, the same perturbation of Vipp1 in dark alone or light alone growth conditions causes no growth or thylakoid morphology defects. We propose that the punctuated dynamics of Vipp1 at the cell periphery in regions of high thylakoid curvature enable acquisition of photosynthetic competency, perhaps by facilitating biogenesis of photosynthetic complexes involved in light-dependent reactions of photosynthesis. In live and growing cyanobacterial cells, cytosolic Vipp1 continuously concentrates on and off at regions of high thylakoid curvature. These punctuated dynamics of Vipp1 represent events of Vipp1 oligomerization on the thylakoid membrane which are essential for turning on photosynthetic metabolism, perhaps by mediating functional assembly of photosynthetic machinery.
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影响因子：
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作者：
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通讯作者：
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