Amniotic Fluid Volume Responses to Intra-Amniotic Infusion of Lactate in Fetal Sheep
Amniotic Fluid Volume Responses to Intra-Amniotic Infusion of Lactate in Fetal Sheep
复制标题
胎羊羊水量对羊膜腔内输注乳酸的反应
DOI：
10.1177/107155760000700203
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
R. Brace
中科院分区：
文献类型：
--
作者：
E. Scheve;R. Brace
目的：在人类和绵羊胎儿中，严重贫血与胎儿血液和羊水乳酸水平升高和羊水过多有关。在绵羊胎儿中，血管内灌注乳酸钠可提高胎儿血浆和羊水乳酸水平，并产生羊水过多。本研究验证了在胎儿血浆乳酸浓度未增加的情况下羊水乳酸浓度升高与羊水体积（AFV）增加相关的假设。方法：对8只长期置管的妊娠晚期胎羊进行了5天以上的研究。我们每天两次测量血气和pH值、电解质、葡萄糖、乳酸和血尿素氮（BUN）浓度，以及胎儿血、母体血、羊水和胎儿尿的渗透压。每日测定羊水量1次。实验第2 ~ 4天，将乳酸注入羊膜腔室，使羊膜乳酸浓度达到约20 mmol/L。统计分析采用方差分析和回归分析。结果：羊水乳酸水平在输注前平均为2.2±0.4 mmol/L（平均±标准误差），输注72 h时平均为18.9±3.3 mmol/L，输注后降至5.8±1.2 mmol/L （P < 0.001）。第1天胎儿血浆乳酸浓度平均为1.8±0.1 mmol/L，第4天升高了1.4±0.6 mmol/L （P < 0.001）。5天内胎儿尿流量不变，平均为0.54±0.08 mL/min。羊膜胎儿体积第1天为821±186 mL，第4天增加99±95 mL，第5天保持不变。结论：本研究提示羊水乳酸通过渗透作用增加AFV，但作用不大。因此，胎儿乳酸水平升高的主要作用位点似乎在胎盘而不是膜内途径。
Objective: In human and ovine fetuses, severe anemia is associated with elevated fetal blood and amniotic lactate levels and polyphydramnios. In ovine fetuses, intravascular infusions of sodium lactate elevates fetal plasma and amniotic lactate levels and produces polyhydramnios. The present study tested the hypothesis that an elevated amniotic lactate concentration in the absence of an increased fetal plasma lactate would be associated with an increase in amniotic fluid volume (AFV). Methods: Eight chronically catheterized, late-gestation fetal sheep were studied over 5 days. Twice each day, we measured blood gases and pH, electrolytes, glucose, lactate, and blood urea nitrogen (BUN) concentrations, as well as osmolality of fetal blood, maternal blood, amniotic fluid, and fetal urine. Amniotic fluid volume was measured once daily. During experimental days 2 to 4, lactic acid was infused into the amniotic compartment to achieve an amniotic lactate concentration of approximately 20 mmol/L. Statistical analysis was by analysis of variance and regression. Results: Amniotic fluid lactate levels averaged 2.2 ± 0.4 mmol/L (mean ± standard error) before infusion and 18.9 ± 3.3 mmol/L (during the 72-hour infusion, falling to 5.8 ± 1.2 mmol/L postinfusion (P < .001). Fetal plasma lactate averaged 1.8 ± 0.1 mmol/L on day 1 and increased by 1.4 ± 0.6 mmol/L on day 4 (P < .001). Fetal urine flow was unchanged and averaged 0.54 ± 0.08 mL/min over the 5 days. Amniotic fetal volume was 821 ± 186 mL on day 1, increased nonsignificantly by 99 ± 95 mL on day 4, and remained unchanged on day 5. Conclusions: The present study suggests that if amniotic lactate acts osmotically to increase AFV, the effect is small. Thus, the primary site of action of elevated fetal lactate levels appears to be at the placenta rather than the intramembranous pathway.
藤壶肌纤维中 Na( )-H 交换的收缩诱导激活。
DOI：
10.1152/ajpcell.1994.266.6.c1744
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Davis,BA;Hogan,EM;Boron,WF
通讯作者：
Boron,WF
影响因子：
7.2
作者：
Gilbert,WM;Brace,RA
通讯作者：
Brace,RA
绵羊胎儿慢性贫血期间的肾功能。
DOI：
10.1152/ajpregu.1994.266.6.r1759
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Davis,LE;Hohimer,AR;Woods,LL
通讯作者：
Woods,LL