Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale.
Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale.
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DOI：
10.1038/s41467-021-23252-3
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发表时间：
2021-05-19
影响因子：
16.6
通讯作者：
Piliarik M
中科院分区：
文献类型：
--
作者：
Robert HML;Holanová K;Bujak Ł;Vala M;Henrichs V;Lánský Z;Piliarik M
空间光调制器已成为先进显微镜的重要工具，可实现3D、相位和超分辨率成像的突破。然而，连续的空间光调制，能够捕捉亚毫秒级的微观运动没有衍射伪影和偏振依赖性是具有挑战性的。在这里，我们提出了一种光热空间光调制器（PT-SLM），能够实现纳米级3D重建的快速相位成像。PT-SLM可以产生阶梯状的波前变化，没有衍射伪影，具有高透射率和独立于光偏振的调制效率。我们实现了相移> π和响应时间短至70 µs，理论极限在亚微秒范围内。我们使用PT-SLM对亚衍射物质进行定量相位成像，以破译微管的3D纳米级位移，并研究扩散性微管相关蛋白的轨迹，从而深入了解蛋白质导航通过复杂微管网络的机制。在这里，作者提出了一种高速光热空间光调制器，它可以产生一个台阶状的波前变化，没有衍射伪影。他们使用它来执行定量相位成像，以纳米分辨率在3D中捕获亚毫秒运动。
Spatial light modulators have become an essential tool for advanced microscopy, enabling breakthroughs in 3D, phase, and super-resolution imaging. However, continuous spatial-light modulation that is capable of capturing sub-millisecond microscopic motion without diffraction artifacts and polarization dependence is challenging. Here we present a photothermal spatial light modulator (PT-SLM) enabling fast phase imaging for nanoscopic 3D reconstruction. The PT-SLM can generate a step-like wavefront change, free of diffraction artifacts, with a high transmittance and a modulation efficiency independent of light polarization. We achieve a phase-shift > π and a response time as short as 70 µs with a theoretical limit in the sub microsecond range. We used the PT-SLM to perform quantitative phase imaging of sub-diffractional species to decipher the 3D nanoscopic displacement of microtubules and study the trajectory of a diffusive microtubule-associated protein, providing insights into the mechanism of protein navigation through a complex microtubule network. Here, the authors present a high-speed photothermal spatial light modulator which can generate a step-like wavefront change without diffraction artifacts. They use this to perform quantitative phase imaging, capturing sub-millisecond motion with a nanometer resolution in 3D.
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影响因子：
5
作者：
Holanova, Kristyna;Vala, Milan;Piliarik, Marek
通讯作者：
Piliarik, Marek
影响因子：
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作者：
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