Cryopreserved human adipose-derived stromal vascular fraction maintains fracture healing potential via angiogenesis and osteogenesis in an immunodeficient rat model.
Cryopreserved human adipose-derived stromal vascular fraction maintains fracture healing potential via angiogenesis and osteogenesis in an immunodeficient rat model.
复制标题
免疫缺陷大鼠模型中冻存的人脂肪源性基质血管成分通过血管生成和成骨维持骨折愈合潜力。
DOI：
10.1186/s13287-021-02182-3
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发表时间：
2021-02-04
影响因子：
7.5
通讯作者：
Matsumoto T
中科院分区：
文献类型：
--
作者：
Kamenaga T;Kuroda Y;Nagai K;Tsubosaka M;Takashima Y;Kikuchi K;Fujita M;Ikuta K;Anjiki K;Maeda T;Nakano N;Takayama K;Hashimoto S;Hayashi S;Matsushita T;Niikura T;Kuroda R;Matsumoto T
骨不连严重影响患者的生活质量，需要新的治疗策略来治疗骨不连。我们评估了局部移植人间质血管细胞（SVF）对大鼠非愈合骨折模型骨折愈合的治疗效果，并比较了新鲜分离(F)和冷冻保存(C)-SVF的效果。采用烧灼骨膜法制备雌性免疫缺陷大鼠（F344/N Jcl rnu/rnu）股骨，建立不愈合骨折模型。未愈合骨折形成后，立即将F和c - svf局部移植于磷酸盐缓冲盐水（PBS）中或相同体积的不含细胞的PBS中，使用相同的支架作为对照组。术后8周，进行影像学、组织学、免疫组织化学和生物力学分析，评估骨折愈合情况。影像学结果比较采用卡方检验，组间免疫组化、组织学和生物力学结果的多重比较采用单因素方差分析。概率值为0.05被认为具有统计学意义。在第8周，60%接受F-SVF细胞治疗的动物和50%接受C-SVF细胞治疗的动物的骨折在放射学上愈合并骨愈合，而对照组则观察到骨不愈合。组织学和生物力学评估也证实了愈合潜力。与对照组相比，F-和C-SVF组内在血管生成/成骨的愈合机制之一得到了增强。体外分化实验也证实，与对照组相比，F-和C-SVF组也增强了人类细胞来源的血管生成/成骨功能，这可能是另一种愈合机制。SVF细胞可以促进骨愈合，并且冷冻保存细胞与新鲜细胞具有几乎相同的潜力。SVF细胞可作为其他间充质干细胞的替代品，用于促进骨不连骨折愈合，并且SVF细胞在低温保存下可以保持其作用。
Novel therapeutic strategies for the healing of nonunion, which has serious effects on the quality of life of patients, are needed. We evaluated the therapeutic effect of local transplantation of human stromal vascular fraction (SVF) cells on fracture healing in a rat non-healing fracture model and compared the effects between freshly isolated (F) and cryopreserved (C)-SVFs. Non-healing fracture model was induced in the femur of female immunodeficient rats (F344/N Jcl rnu/rnu) with cauterizing periosteum. Immediately after the creation of non-healing fracture, rats received local transplantation of F and C-SVFs suspended in phosphate-buffered saline (PBS) or the same volume of PBS without cells using the same scaffold as a control group. During 8 weeks post-surgery, radiologic, histological, immunohistochemical, and biomechanical analyses were performed to evaluate fracture healing. The comparison of radiological results was performed with a chi-square test, and the multiple comparisons of immunohistochemical, histological, and biomechanical results among groups were made using a one-way analysis of variance. A probability value of 0.05 was considered to denote statistical significance. At week 8, in 60% of animals receiving F-SVF cells and in 50% of animals receiving C-SVF cells, the fracture radiologically healed with bone union whereas nonunion was observed in the control group. The healing potential was also confirmed by histological and biomechanical assessments. One of the mechanisms underlying healing involving intrinsic angiogenesis/osteogenesis was enhanced in F- and C-SVF groups compared with that in the control group. Human cell-derived vasculogenesis/osteogenesis, which was also confirmed in an in vitro differentiation assay, was also enhanced in the F- and C-SVF groups compared with that in the control groups and could be another mechanism for healing. SVF cells can enhance bone healing and cryopreserved cells have almost equal potential as fresh cells. SVF cells can be used for improving nonunion bone fracture healing as an alternative to other mesenchymal stem cells and the effect of SVF cells can be maintained under cryopreservation.
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影响因子：
4.8
作者：
Inokuchi, Takao;Matsumoto, Tomoyuki;Kuroda, Ryosuke
通讯作者：
Kuroda, Ryosuke
DOI：
10.1093/ndt/gfq603
发表时间：
2010-12
期刊：
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
影响因子：
--
作者：
Feng Z;Ting J;Alfonso Z;Strem BM;Fraser JK;Rutenberg J;Kuo HC;Pinkernell K
通讯作者：
Pinkernell K
影响因子：
3
作者：
De Rosa, Alfredo;De Francesco, Francesco;Papaccio, Gianpaolo
通讯作者：
Papaccio, Gianpaolo
DOI：
10.3109/17453678008990848
发表时间：
1980-01-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
ALLEN, HL;WASE, A;BEAR, WT
通讯作者：
BEAR, WT
影响因子：
2
作者：
Alatab S;Shekarchian S;Najafi I;Moghadasali R;Ahmadbeigi N;Pourmand MR;Bolurieh T;Jaroughi N;Pourmand G;Aghdami N
通讯作者：
Aghdami N