Immune responses elicited by bacterial minicells capable of simultaneous DNA and protein antigen delivery.
Immune responses elicited by bacterial minicells capable of simultaneous DNA and protein antigen delivery.
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DOI：
10.1016/j.vaccine.2006.04.063
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发表时间：
2006-08-14
期刊：
影响因子：
5.5
通讯作者：
McGuire KL
中科院分区：
文献类型：
--
作者：
Giacalone MJ;Sabbadini RA;Chambers AL;Pillai S;Berkley NL;Surber MW;McGuire KL
最近围绕新出现的传染病、生物恐怖主义和细菌对多药抗生素耐药性增加的事件大大增加了目前对有效疫苗的需求。多年的研究表明，活的、减毒的病原体往往更有效地递送异源蛋白或DNA，以诱导保护性免疫反应。然而，这些疫苗载体存在固有的安全性问题，限制了它们的开发和在许多患者群体中的使用。使用非活体递送机制的研究表明，同时向个体提供蛋白质抗原和编码抗原的DNA可诱导更好的、更具保护性的免疫反应，但很少同时递送。在这里，来自共生大肠杆菌菌株的非复制细菌小细胞在同时传递蛋白质和DNA后有效地诱导抗原特异性免疫反应。这些数据证明了染色体细菌小细胞作为疫苗载体的潜在用途。
Recent events surrounding emerging infectious diseases, bioterrorism and increasing multidrug antibiotic resistance in bacteria have drastically increased current needs for effective vaccines. Many years of study have shown that live, attenuated pathogens are often more effective at delivering heterologous protein or DNA to induce protective immune responses. However, these vaccine carriers have inherent safety concerns that have limited their development and their use in many patient populations. Studies using nonliving delivery mechanisms have shown that providing both protein antigen and DNA encoding the antigen to an individual induces an improved, more protective immune response but rarely, if ever, are both delivered simultaneously. Here, non-replicating bacterial minicells derived from a commensal E. coli strain are shown to effectively induce antigen-specific immune responses after simultaneous protein and DNA delivery. These data demonstrate the potential use of achromosomal bacterial minicells as a vaccine carrier.
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影响因子：
2.7
作者：
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