Robust cre-mediated recombination in small intestinal stem cells utilizing the olfm4 locus.
Robust cre-mediated recombination in small intestinal stem cells utilizing the olfm4 locus.
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DOI：
10.1016/j.stemcr.2014.05.018
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发表时间：
2014-08-12
影响因子：
5.9
通讯作者：
Clevers, Hans
中科院分区：
文献类型：
--
作者：
Schuijers, Jurian;van der Flier, Laurens G.;van Es, Johan;Clevers, Hans
小肠上皮是哺乳动物中自我更新最快的组织。我们先前证明了在肠隐窝底部存在由Lgr 5表达定义的长寿命循环干细胞池。Lgr 5-eGFP-IRES-CreERT 2敲入等位基因在表征和分析这些细胞方面发挥了重要作用，但其低水平表达及其在邻近隐窝斑块中的沉默并不允许定量基因缺失。嗅觉介导蛋白-4（Olfm 4）已经从Lgr 5干细胞的基因签名中出现，作为鼠小肠干细胞的稳健标志物。我们观察到Olfm 4缺失动物没有表现出表型，并报道了Olfm 4-IRES-eGFPCreERT 2敲入小鼠模型的产生，该模型允许小肠上皮中Lgr 5+干细胞的可视化和遗传操作。eGFPCreERT 2融合蛋白忠实地标记小肠中的所有干细胞，并以强大的效率诱导条件LacZ报告基因的激活。Olfm 4的缺失不会导致表型Olfm 4-IRES-eGFPCreERT 2在体内强烈激活LacZ报告基因Olfm 4驱动的eGFPCreERT 2表达标志着小肠的干细胞Clevers和同事报告说Olfm 4缺失动物没有表现出表型，并报道了Olfm 4-IRES-eGFPCreERT 2敲入小鼠模型的产生，该模型允许可视化和遗传操作Lgr 5 +小肠上皮中的干细胞。eGFPCreERT 2融合蛋白忠实地标记小肠中的所有干细胞，并以强大的效率诱导条件LacZ报告基因的激活。
The epithelium of the small intestine is the most rapidly self-renewing tissue in mammals. We previously demonstrated the existence of a long-lived pool of cycling stem cells defined by Lgr5 expression at the bottom of intestinal crypts. An Lgr5-eGFP-IRES-CreERT2 knockin allele has been instrumental in characterizing and profiling these cells, yet its low level expression and its silencing in patches of adjacent crypts have not allowed quantitative gene deletion. Olfactomedin-4 (Olfm4) has emerged from a gene signature of Lgr5 stem cells as a robust marker for murine small intestinal stem cells. We observe that Olfm4null animals show no phenotype and report the generation of an Olfm4-IRES-eGFPCreERT2 knockin mouse model that allows visualization and genetic manipulation of Lgr5+ stem cells in the epithelium of the small intestine. The eGFPCreERT2 fusion protein faithfully marks all stem cells in the small intestine and induces the activation of a conditional LacZ reporter with robust efficiency. Deletion of Olfm4 does not lead to a phenotype Olfm4-IRES-eGFPCreERT2 robustly activates a LacZ reporter in vivo Olfm4-driven eGFPCreERT2 expression marks the stem cells of the small intestine Clevers and colleagues report that Olfm4null animals show no phenotype, and report the generation of an Olfm4-IRES-eGFPCreERT2 knockin mouse model that allows visualization and genetic manipulation of Lgr5+ stem cells in the epithelium of the small intestine. The eGFPCreERT2 fusion protein faithfully marks all stem cells in the small intestine and induces the activation of a conditional LacZ reporter with robust efficiency.
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影响因子：
64.5
作者：
Powell AE;Wang Y;Li Y;Poulin EJ;Means AL;Washington MK;Higginbotham JN;Juchheim A;Prasad N;Levy SE;Guo Y;Shyr Y;Aronow BJ;Haigis KM;Franklin JL;Coffey RJ
通讯作者：
Coffey RJ
影响因子：
64.8
作者：
Buczacki, Simon J. A.;Zecchini, Heather Ireland;Winton, Douglas J.
通讯作者：
Winton, Douglas J.
影响因子：
5.3
作者：
Morita, H;Mazerbourg, S;Hsueh, AJW
通讯作者：
Hsueh, AJW
DOI：
10.1111/j.1365-2184.1974.tb00907.x
发表时间：
1974-01-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
--
作者：
POTTEN, CS;KOVACS, L;HAMILTON, E
通讯作者：
HAMILTON, E
影响因子：
29.4
作者：
Gregorieff, A;Pinto, D;Clevers, H
通讯作者：
Clevers, H