Prejunctional Muscarinic Receptors Modulating Acetylcholine Release in Rabbit Detrusor Smooth Muscles
Prejunctional Muscarinic Receptors Modulating Acetylcholine Release in Rabbit Detrusor Smooth Muscles
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结合前毒蕈碱受体调节兔逼尿肌平滑肌乙酰胆碱的释放
DOI：
10.1159/000030309
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发表时间：
1999
影响因子：
1.6
通讯作者：
S. Ueda
中科院分区：
文献类型：
--
作者：
A. Inadome;Masaki Yoshida;W. Takahashi;M. Yono;H. Seshita;Y. Miyamoto;T. Kawano;S. Ueda
我们用高效液相色谱结合微透析的方法研究了兔逼尿肌肌条胆碱能神经末梢中功能前受体的存在及其亚型。观察不同药物预处理对乙酰胆碱(ACh)释放和电场刺激引起的收缩反应的影响。阿托品(非选择性M受体拮抗剂)和4-DAMP(M_3受体拮抗剂)对ACh释放无明显影响，但显著降低收缩反应。M1拮抗剂哌仑西平可降低ACh的释放和收缩反应。M2受体拮抗剂美托卡明可增加ACh的释放，但不影响收缩反应。这些结果表明，兔逼尿肌的M受体是异质性的，既有交感前易化受体(M1受体)，又有ACh释放的抑制受体(M2受体)和介导收缩反应的交感后M3受体。
We investigated the presence and subtypes of functionally prejunctional receptors in cholinergic nerve endings of rabbit detrusor smooth muscle strips using high-performance liquid chromatography coupled with a microdialysis procedure. The effects of pretreatment with various drugs on acetylcholine (ACh) release and contractile responses induced by electrical field stimulation were evaluated. Although atropine (a muscarinic nonselective antagonist) and 4-DAMP (a muscarinic M3 antagonist) did not influence the ACh release, they markedly reduced the contractile responses. Pirenzepine (M1 antagonist) decreased ACh release and contractile responses. Methoctramine (M2 antagonist) increased the ACh release, but did not influence to the contractile responses. These results suggest that the muscarinic receptors in the rabbit detrusor smooth muscle are heterogeneous, prejunctional facilitatory (M1 receptors), and inhibitory (M2 receptors) for ACh release and postjunctional M3 receptors mediating contractile responses.
马气道胆碱能神经的乙酰胆碱释放：刺激强度和肌肉预负荷的影响。
DOI：
10.1152/ajplung.1993.264.3.l269
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Wang,Z;Robinson,NE;Yu,M
通讯作者：
Yu,M
DOI：
--
发表时间：
1995-05
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
P. Wang;G. Luthin;M. Ruggieri
通讯作者：
P. Wang;G. Luthin;M. Ruggieri
大鼠膀胱胆碱能神经末梢中抑制性烟碱和促进性毒蕈碱受体的证据。
DOI：
10.1016/0165-1838(92)90237-b
发表时间：
1992
期刊：
Journal of the autonomic nervous system
影响因子：
--
作者：
Somogyi,GT;deGroat,WC
通讯作者：
deGroat,WC