Total chemical synthesis of dengue 2 virus capsid protein via native chemical ligation: role of the conserved salt-bridge.
Total chemical synthesis of dengue 2 virus capsid protein via native chemical ligation: role of the conserved salt-bridge.
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通过天然化学连接进行登革热 2 病毒衣壳蛋白的全化学合成：保守盐桥的作用。
DOI：
10.1016/j.bmc.2013.04.035
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发表时间：
2013
影响因子：
3.5
通讯作者：
Lu,Wuyuan
中科院分区：
文献类型：
--
作者：
Zhan,Changyou;Zhao,Le;Chen,Xishan;Lu,Wei-Yue;Lu,Wuyuan
The dengue capsid protein C is a highly basic alpha-helical protein of ∼100 amino acid residues that forms an emphipathic homodimer to encapsidate the viral genome and to interact with viral membranes. The solution structure of dengue 2 capsid protein C (DEN2C) has been determined by NMR spectroscopy, revealing a large dimer interface formed almost exclusively by hydrophobic residues. The only acidic residue (Glu87) conserved in the capsid proteins of all four serotypes of dengue virus forms a salt bridge with the side chains of Lys45 and Arg55′. To understand the structural and functional significance of this conserved salt bridge, we chemically synthesized an N-terminally truncated form of DEN2C (WTDEN2C) and its salt bridge-void analogE87ADEN2C using the native chemical ligation technique developed by Kent and colleagues. Comparative biochemical and biophysical studies of these two synthetic proteins using circular dichroism spectroscopy, fluorescence polarization, protein thermal denaturation, and proteolytic susceptibility assay demonstrated that the conserved salt bridge contributed to DEN2C dimerization and stability as well as its resistance to proteolytic degradation. Our work provided insight into the role of a fully conserved structural element of the dengue capsid protein C and paved the way for additional functional studies of this important viral protein.
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影响因子：
15
作者：
Yan, LZ;Dawson, PE
通讯作者：
Dawson, PE
影响因子：
1.8
作者：
Johnson, Erik C. B.;Kent, Stephen B. H.
通讯作者：
Kent, Stephen B. H.
从防御素原到防御素：人中性粒细胞α-防御素原-1 及其成熟结构域的合成和表征。
DOI：
10.1034/j.1399-3011.2003.00068.x
发表时间：
2003
期刊：
The journal of peptide research : official journal of the American Peptide Society
影响因子：
--
作者：
Z. Wu;A. Prahl;R. Powell;Bryan Ericksen;J. Lubkowski;W. Lu
通讯作者：
W. Lu
影响因子：
15
作者：
Johnson, Erik C. B.;Kent, Stephen B. H.
通讯作者：
Kent, Stephen B. H.
影响因子：
3.7
作者：
Markoff, L;Falgout, B;Chang, A
通讯作者：
Chang, A