On-command on/off switching of progenitor cell and cancer cell polarized motility and aligned morphology via a cytocompatible shape memory polymer scaffold.
On-command on/off switching of progenitor cell and cancer cell polarized motility and aligned morphology via a cytocompatible shape memory polymer scaffold.
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祖细胞和癌细胞极化运动的命令开/关开关，并通过细胞相容形状的记忆聚合物支架对齐形态。
DOI：
10.1016/j.biomaterials.2017.06.016
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发表时间：
2017-09
期刊：
影响因子：
14
通讯作者：
Henderson JH
中科院分区：
文献类型：
--
作者：
Wang J;Quach A;Brasch ME;Turner CE;Henderson JH
体外生物材料模型在理解细胞外基质 (ECM) 结构在细胞运动和极性控制中的作用方面取得了进展。然而，大多数模型是静态的，无法模拟体内 ECM 重塑和功能的动态方面。为了解决这一限制，我们提出了一种电纺形状记忆聚合物支架，它可以在细胞相容性条件下根据命令改变纤维排列。使用人纤维肉瘤细胞系 HT-1080 和鼠间充质干细胞系 C3H/10T1/2 研究细胞反应。结果表明，细胞极化运动和排列的按命令开/关切换成功。纤维排列的减少导致沿着纤维排列方向从偏振运动到非偏振运动以及从排列到未排列形态的变化，而纤维排列的增加导致沿着纤维排列方向从非偏振到偏振运动以及从未排列到排列形态的变化。此外，这些发现与纤维排列增加导致细胞速度增加，而纤维排列减少导致细胞速度降低的假设是一致的。细胞极化运动和排列的按命令开/关切换预计将能够实现肿瘤转移、细胞归巢和组织工程中定向细胞运动的新研究。
In vitro biomaterial models have enabled advances in understanding the role of extracellular matrix (ECM) architecture in the control of cell motility and polarity. Most models are, however, static and cannot mimic dynamic aspects of in vivo ECM remodeling and function. To address this limitation, we present an electrospun shape memory polymer scaffold that can change fiber alignment on command under cytocompatible conditions. Cellular response was studied using the human fibrosarcoma cell line HT-1080 and the murine mesenchymal stem cell line C3H/10T1/2. The results demonstrate successful on-command on/off switching of cell polarized motility and alignment. Decrease in fiber alignment causes a change from polarized motility along the direction of fiber alignment to non-polarized motility and from aligned to unaligned morphology, while increase in fiber alignment causes a change from non-polarized to polarized motility along the direction of fiber alignment and from unaligned to aligned morphology. In addition, the findings are consistent with the hypothesis that increased fiber alignment causes increased cell velocity, while decreased fiber alignment causes decreased cell velocity. On-command on/off switching of cell polarized motility and alignment is anticipated to enable new study of directed cell motility in tumor metastasis, in cell homing, and in tissue engineering.
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