Sorting cells by their dynamical properties.
Sorting cells by their dynamical properties.
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DOI：
10.1038/srep34375
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发表时间：
2016-10-06
影响因子：
4.6
通讯作者：
Gompper G
中科院分区：
文献类型：
--
作者：
Henry E;Holm SH;Zhang Z;Beech JP;Tegenfeldt JO;Fedosov DA;Gompper G
细胞分选的最新进展旨在开发对细胞各种机械特性敏感的新方法。微流控技术在细胞分选方面具有巨大的潜力；然而，许多微器件的设计是基于以尺寸作为分离参数的刚性球形颗粒的理论。显然，大多数生物颗粒都是非球形且可变形的，因此在流体流动中表现出比刚性球体更复杂的行为。在这里，我们通过采用中尺度流体动力学模拟和微流体实验的结合，展示了细胞的力学和动力学特性作为分离的生物标志物。研究了红细胞（红细胞）在确定性横向位移（DLD）装置中的动态行为，研究了不同装置几何形状和细胞内流体和悬浮介质之间的粘度对比。我们发现黏度对比和相关的细胞动力学清楚地决定红细胞轨迹通过DLD装置。模拟结果与实验结果比较好，为DLD器件中非球形和可变形细胞分选的物理机制提供了新的见解。最后，我们讨论了基于细胞大小以外的特性（如力学和形态学）的细胞动力学对分选方案的影响。
Recent advances in cell sorting aim at the development of novel methods that are sensitive to various mechanical properties of cells. Microfluidic technologies have a great potential for cell sorting; however, the design of many micro-devices is based on theories developed for rigid spherical particles with size as a separation parameter. Clearly, most bioparticles are non-spherical and deformable and therefore exhibit a much more intricate behavior in fluid flow than rigid spheres. Here, we demonstrate the use of cells’ mechanical and dynamical properties as biomarkers for separation by employing a combination of mesoscale hydrodynamic simulations and microfluidic experiments. The dynamic behavior of red blood cells (RBCs) within deterministic lateral displacement (DLD) devices is investigated for different device geometries and viscosity contrasts between the intra-cellular fluid and suspending medium. We find that the viscosity contrast and associated cell dynamics clearly determine the RBC trajectory through a DLD device. Simulation results compare well to experiments and provide new insights into the physical mechanisms which govern the sorting of non-spherical and deformable cells in DLD devices. Finally, we discuss the implications of cell dynamics for sorting schemes based on properties other than cell size, such as mechanics and morphology.
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