Cytokine expression profile over time in burned mice.
Cytokine expression profile over time in burned mice.
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DOI：
10.1016/j.cyto.2008.10.005
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发表时间：
2009-01
期刊：
影响因子：
3.8
通讯作者：
Jeschke, Marc G.
中科院分区：
文献类型：
--
作者：
Finnerty, Celeste C.;Przkora, Rene;Herndon, David N.;Jeschke, Marc G.
严重烧伤引起的持续性炎症反应增加了患者对感染和脓毒症的易感性，有可能导致多器官衰竭和死亡。为了利用小鼠模型开发调节烧伤后炎症反应的干预措施，必须首先确定小鼠的反应以及与人类反应的相似性。在这里，我们展示了烧伤时血清细胞因子的表达谱，并与假手术小鼠和人类烧伤患者进行了比较。雄性C57BL/6小鼠随机分为对照组(n=47)和烫伤组(n=89)。分别于伤后3、6、9、12、24、48h、7、10、14d处死小鼠，用多重阵列检测细胞因子。烧伤后IL-6、IL-1β、KC、G-CSF、肿瘤坏死因子、IL-17、MIP-1α、RANTES、GM-CSF均升高，P<0.05。IL-2、IL-3、IL-5降低，P<0.05。IL-10、干扰素-γ和IL-12p70呈双相表达，P<0.05。这种暂时性细胞因子的表达模式阐明了烧伤小鼠炎症反应的发病机制。11种细胞因子在小鼠和儿童中的表达相似，在烧伤后第14天恢复到最低水平，证实了烧伤小鼠模型用于开发治疗干预措施以减轻烧伤后炎症反应的有效性。
The persistent inflammatory response induced by a severe burn increases patient susceptibility to infections and sepsis, potentially leading to multi-organ failure and death. In order to use murine models to develop interventions that modulate the post-burn inflammatory response, the response in mice and the similarities to the human response must first be determined. Here we present the temporal serum cytokine expression profiles in burned in comparison to sham mice and human burn patients. Male C57BL/6 mice were randomized to control (n=47) or subjected to a 35% TBSA scald burn (n=89). Mice were sacrificed 3, 6, 9, 12, 24, 48 hours and 7, 10, and 14 days post-burn; cytokines were measured by multi-plex array. Following the burn injury, IL-6, IL-1β, KC, G-CSF, TNF, IL-17, MIP-1α, RANTES, and GM-CSF were increased, p<0.05. IL-2, IL-3, and IL-5 were decreased, p<0.05. IL-10, IFN-γ, and IL-12p70 were expressed in a biphasic manner, p<0.05. This temporal cytokine expression pattern elucidates the pathogenesis of the inflammatory response in burned mice. Expression of 11 cytokines were similar in mice and children, returning to lowest levels by post-burn day 14, confirming the utility of the burned mouse model for development of therapeutic interventions to attenuate the post-burn inflammatory response.
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影响因子：
15.1
作者：
Finnerty, Celeste C.;Herndon, David N.;Jeschke, Marc G.
通讯作者：
Jeschke, Marc G.
影响因子：
15.1
作者：
Jeschke, Marc G.;Mlcak, Ronald P.;Finnerty, Celeste C.;Norbury, William B.;Gauglitz, Gerd G.;Kulp, Gabriela A.;Herndon, David N.
通讯作者：
Herndon, David N.
影响因子：
4.4
作者：
Ipaktchi, Kyros;Mattar, Aladdein;Arbabi, Saman
通讯作者：
Arbabi, Saman
影响因子：
5.7
作者：
Jeschke, MG;Einspanier, R;Jauch, KW
通讯作者：
Jauch, KW
影响因子：
3.1
作者：
Finnerty, Celeste C.;Herndon, David N.;Jeschke, Marc G.
通讯作者：
Jeschke, Marc G.