Fluorocarbon Exposure Mode Markedly Affects Phospholipid Monolayer Behavior at the Gas/Liquid Interface: Impact on Size and Stability of Microbubbles.
Fluorocarbon Exposure Mode Markedly Affects Phospholipid Monolayer Behavior at the Gas/Liquid Interface: Impact on Size and Stability of Microbubbles.
复制标题
氟碳暴露模式显着影响气/液界面处的磷脂单层行为：对微泡尺寸和稳定性的影响。
DOI：
10.1021/acs.langmuir.8b03546
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
M. Krafft
中科院分区：
文献类型：
--
作者：
Danjie Shi;Xianhe Liu;C. Counil;M. Krafft
尽管大多数用于医疗应用的磷脂微泡(MBS)是由氟碳(FC)气体稳定的，但关于磷脂和FC分子在气/水界面上相互作用的信息仍然很少。我们报道了全氟己烷(F-己烷)的引入过程，即在二甲基磷脂酰胆碱(DMPC)或二棕榈酰磷脂酰胆碱(DPPC)Langmuir单分子膜上方的气相中，或者在水相中，从根本上影响了压缩等温线。当引入气相时，F-己烷迅速结合到界面膜中，但也很容易在压缩时解吸，最终完全从磷脂单分子层中排出。相反，当F-己烷被引入水相时，它仍然被困在界面上。这些不同的结果表明，磷脂单分子层起到了屏障的作用，有效地阻止了FC在界面膜上的转移。气泡轮廓分析表明，F-己烷能显著加快磷脂在气/水界面的吸附动力学，降低界面张力。当F-己烷从气相中提供时，这些影响的程度更加明显。DMPC和DPPC壳层微泡的大小和稳定性也取决于FC的引入方式。与仅在氮气中制备的参考MBS相比，F-己烷的引入总是导致MB平均半径的减小。然而，尽管对于DMPC来说，这种下降取决于F-己烷的引入程序，但它与程序无关，对于DPPC来说最明显的是(从∼2.0μm到∼1.0μm)。在气相中引入FC对甲基溴的半衰期的影响最大(dmpc和dppc的t1/2分别为∼1.8h和6.8h)，而通过水相输送时的t1/2分别为∼0.8h和∼1.7h。无氟碳参照物DMPC和DPPC气泡的半衰期分别为∼0.5h和0.8h。从磷脂与F-己烷的相互作用和单层流态化效应两个方面讨论了F-己烷对MB特性的影响，如Langmuir和张力计研究所揭示的那样。
Although most phospholipid-shelled microbubbles (MBs) investigated for medical applications are stabilized by a fluorocarbon ( FC) gas, information on the interactions between the phospholipid and FC molecules at the gas/water interface remains scarce. We report that the procedure of introduction of perfluorohexane ( F-hexane), that is, either in the gas phase above dimyristoylphosphatidylcholine (DMPC) or dipalmitoylphosphatidylcholine (DPPC) Langmuir monolayers, or in the aqueous subphase, radically affects the compression isotherms. When introduced in the gas phase, F-hexane is rapidly incorporated in the interfacial film, but is also readily desorbed upon compression and eventually totally expelled from the phospholipid monolayers. By contrast, when introduced in the aqueous phase, F-hexane remains trapped at the interface. These dissimilar outcomes demonstrate that the phospholipid monolayer acts as a barrier that effectively hinders the transfer of the FC across the interfacial film. F-hexane was also found to significantly accelerate the adsorption kinetics of the phospholipids at the gas/water interface and to lower the interfacial tension, as assessed by bubble profile analysis tensiometry. The extent of these effects is more pronounced when F-hexane is provided from the gas phase. The size and stability characteristics of DMPC- and DPPC-shelled microbubbles were also found to depend on how the FC is introduced. As compared to reference MBs prepared under nitrogen only, introduction of F-hexane always causes a decrease in MB mean radius. However, while for DMPC this decrease depends on the F-hexane introduction procedure, it is independent from the procedure and most pronounced (from ∼2.0 μm to ∼1.0 μm) for DPPC. Introducing the FC in the gas phase has the strongest effect on MB half-life ( t1/2 = ∼1.8 and 6.8 h for DMPC and DPPC, respectively), as compared to when it is delivered through the aqueous phase (∼0.8 and ∼1.7 h). Fluorocarbonless reference DMPC and DPPC bubbles had a half-life of ∼0.5 and 0.8 h, respectively. The effects of F-hexane on MB characteristics are discussed with regard to the interactions between phospholipids and F-hexane and monolayer fluidization effect, as revealed by the Langmuir and tensiometric studies.
登录
查看更多内容
影响因子：
18.3
作者：
Ferrara, Katherine W.;Borden, Mark A.;Zhang, Hua
通讯作者：
Zhang, Hua
影响因子：
16.1
作者：
Sirsi, Shashank R.;Borden, Mark A.
通讯作者：
Borden, Mark A.
影响因子：
2.9
作者：
Sarkar, Kausik;Katiyar, Amit;Jain, Pankaj
通讯作者：
Jain, Pankaj
影响因子：
3.9
作者：
Talu, Esra;Hettiarachchi, Kanaka;Longo, Marjorie L.
通讯作者：
Longo, Marjorie L.
影响因子：
9.9
作者：
Katiyar, Amit;Sarkar, Kausik;Jain, Pankaj
通讯作者：
Jain, Pankaj