Sensory rhodopsin II from the haloalkaliphilic natronobacterium pharaonis: light-activated proton transfer reactions.
Sensory rhodopsin II from the haloalkaliphilic natronobacterium pharaonis: light-activated proton transfer reactions.
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来自卤碱嗜碱杆菌 pharaonis 的感觉视紫红质 II：光激活质子转移反应。
DOI：
10.1016/s0006-3495(00)76654-9
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发表时间：
2000
影响因子：
3.4
通讯作者：
E. Bamberg
中科院分区：
文献类型：
--
作者：
G. Schmies;B. Lüttenberg;I. Chizhov;Martin Engelhard;A. Becker;E. Bamberg
本工作采用闪光光解和黑脂膜（black lipid membrane，BLM）技术研究了感觉型pharaonis盐杆菌视紫红质II（pSRII）及其通道突变体D 75 N-pSRII和F86 D-pSRII的光活化质子转移反应。F86 D-pSRII突变体的光循环与野生型蛋白非常相似，而D 75 N-pSRII的光循环仅由两个中间体组成。加入外部质子供体如叠氮化物，或在F86 D-pSRII的情况下，咪唑，加速了席夫碱的再质子化，但不加速转换。电测量证明，pSRII和F86 D-pSRII可以作为向外定向的质子泵，而细胞外通道（D 75 N-pSRII）的突变导致向内定向的瞬态电流。光稳电流的大小几乎可以忽略不计，这可以用大约一秒的长时间光循环来解释。虽然M衰变，但不是光循环周转，pSRII和F86 D-pSRII的加速通过添加叠氮化物，光稳电流大大增加。它被讨论，在一个双光子过程中的后期中间体（N-和/或O-类物种）被光转换回原来的静止状态，从而长的光循环被切断短，引起的光稳电流的大幅增加。在这项工作中提出的结果表明，功能，以产生跨膜离子梯度是古细菌视紫红质的一般属性。
In the present work the light-activated proton transfer reactions of sensory rhodopsin II fromNatronobacterium pharaonis(pSRII) and those of the channel-mutants D75N-pSRII and F86D-pSRII are investigated using flash photolysis and black lipid membrane (BLM) techniques. Whereas the photocycle of the F86D-pSRII mutant is quite similar to that of the wild-type protein, the photocycle of D75N-pSRII consists of only two intermediates. The addition of external proton donors such as azide, or in the case of F86D-pSRII, imidazole, accelerates the reprotonation of the Schiff base, but not the turnover. The electrical measurements prove that pSRII and F86D-pSRII can function as outwardly directed proton pumps, whereas the mutation in the extracellular channel (D75N-pSRII) leads to an inwardly directed transient current. The almost negligible size of the photostationary current is explained by the long-lasting photocycle of about a second. Although the M decay, but not the photocycle turnover, of pSRII and F86D-pSRII is accelerated by the addition of azide, the photostationary current is considerably increased. It is discussed that in a two-photon process a late intermediate (N- and/or O-like species) is photoconverted back to the original resting state; thereby the long photocycle is cut short, giving rise to the large increase of the photostationary current. The results presented in this work indicate that the function to generate ion gradients across membranes is a general property of archaeal rhodopsins.
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影响因子：
2.9
作者：
BRAIMAN, MS;MOGI, T;ROTHSCHILD, KJ
通讯作者：
ROTHSCHILD, KJ
测试 BR 光循环动力学。
DOI：
10.1016/s0006-3495(95)80321-8
发表时间：
1995
期刊：
Biophysical journal.
影响因子：
--
作者：
Nagle,JF;Zimanyi,L;Lanyi,JK
通讯作者：
Lanyi,JK
影响因子：
3.4
作者：
J. Sasaki;J. Spudich
通讯作者：
J. Sasaki;J. Spudich
DOI：
10.1073/pnas.91.21.10188
发表时间：
1994-10-11
影响因子：
11.1
作者：
BOGOMOLNI, RA;STOECKENIUS, W;SPUDICH, JL
通讯作者：
SPUDICH, JL
DOI：
10.1073/pnas.94.10.4960
发表时间：
1997-05-13
影响因子：
11.1
作者：
Spudich, EN;Zhang, WS;Spudich, JL
通讯作者：
Spudich, JL