Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1.
Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1.
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DOI：
10.1186/s12870-014-0227-1
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发表时间：
2014-08-20
影响因子：
5.3
通讯作者：
Doohan FM
中科院分区：
文献类型：
--
作者：
Ali SS;Gunupuru LR;Kumar GB;Khan M;Scofield S;Nicholson P;Doohan FM
类胡萝卜素激素调节植物生长和发育的许多方面。膜受体BRI 1是油菜素类固醇信号级联中的核心参与者。半矮秆'uzu'大麦在BRI 1的激酶尾的保守结构域中携带突变，并且该突变等位基因因其对产量和抗倒伏性的积极贡献而被认可。在这里，我们表明，涡大麦表现出增强的抵抗力，一系列病原体。这是由于预先形成的，诱导型和组成型防御反应的组合，如通过转录组学和生物化学研究的组合所确定的。基因表达研究用于确定uzu衍生物在下游油菜素类固醇信号传导中减弱。通过病毒诱导的基因沉默降低BRI 1 RNA水平损害了对日本风病的抗性。毒素衍生物的病原体抗性可能是由于BRI 1的多效性效应或其抑制的BR信号传导的级联效应。本文的在线版本（doi：10.1186/s12870-014-0227-1）包含补充材料，可供授权用户使用。
Brassinosteroid hormones regulate many aspects of plant growth and development. The membrane receptor BRI1 is a central player in the brassinosteroid signaling cascade. Semi-dwarf ‘uzu’ barley carries a mutation in a conserved domain of the kinase tail of BRI1 and this mutant allele is recognised for its positive contribution to both yield and lodging resistance. Here we show that uzu barley exhibits enhanced resistance to a range of pathogens. It was due to a combination of preformed, inducible and constitutive defence responses, as determined by a combination of transcriptomic and biochemical studies. Gene expression studies were used to determine that the uzu derivatives are attenuated in downstream brassinosteroid signaling. The reduction of BRI1 RNA levels via virus-induced gene silencing compromised uzu disease resistance. The pathogen resistance of uzu derivatives may be due to pleiotropic effects of BRI1 or the cascade effects of their repressed BR signaling. The online version of this article (doi:10.1186/s12870-014-0227-1) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Imbeaud S;Graudens E;Boulanger V;Barlet X;Zaborski P;Eveno E;Mueller O;Schroeder A;Auffray C
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