Insulin promotes progression of colon cancer by upregulation of ACAT1.
Insulin promotes progression of colon cancer by upregulation of ACAT1.
复制标题
胰岛素通过上调 ACAT1 促进结肠癌的进展
DOI：
10.1186/s12944-018-0773-x
复制
发表时间：
2018-05-24
影响因子：
4.5
通讯作者：
Cao D
中科院分区：
文献类型：
--
作者：
Chen X;Liang H;Song Q;Xu X;Cao D
Insulin resistant and the progression of cancer is closely related. The aim of this study was to investigate the effect of insulin on the proliferation and migration of colon cancer cells and its underlying mechanism. Colon carcinoma tissues from the 80 cases of colon cancer patients were collected. Immunohistochemistry was used to detect the expression of acyl coenzyme A: cholesterol acyltransferase1 (ACAT1), and we analyzed the correlation between hyperglycemia and ACAT1, hyperglycemia and metastasis. CCK8 assay and transwell assay were used to investigate the effect of different concentrations of insulin and ACAT1siRNA on human colon cancer cell line HT-29. ACAT1 mRNA expression and protein level in HT-29 cells were determined by real-time quantitative PCR and western blotting, respectively. Biopsies from patients with colon carcinoma showed hyperglycemia links ACAT1, lymph nodes metastasis and distant metastasis. Insulin markedly promoted cell proliferation and migration in human colon cancer HT-29 cells. Moreover, ACAT1mRNA expression and protein level were increased by insulin. ACAT1siRNA resulted in a complete inhibition of the ACAT1 mRNA expression. Consequently insulin-triggered cell proliferation and migration on colon cancer cells were inhibited. The progression of colon cancer has a positive correlation with hyperinsulinemia. Insulin-triggered cell proliferation and metastatic effects on colorectal cancer cells are mediated by ACAT1. Therefore, insulin could promote colon cancer progression by upregulation of ACAT1, which maybe is a potential therapeutic target for colon cancer.
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DOI：
10.1016/j.jsbmb.2014.09.008
发表时间：
2015-07
期刊：
The Journal of steroid biochemistry and molecular biology
影响因子：
--
作者：
Rogers MA;Liu J;Song BL;Li BL;Chang CC;Chang TY
通讯作者：
Chang TY
影响因子：
4.2
作者：
Shibuya Y;Niu Z;Bryleva EY;Harris BT;Murphy SR;Kheirollah A;Bowen ZD;Chang CCY;Chang TY
通讯作者：
Chang TY
影响因子：
29
作者：
Yue S;Li J;Lee SY;Lee HJ;Shao T;Song B;Cheng L;Masterson TA;Liu X;Ratliff TL;Cheng JX
通讯作者：
Cheng JX
影响因子：
3.4
作者：
Bu, Wu-jie;Song, Lei;Liu, Jing
通讯作者：
Liu, Jing
影响因子：
6.2
作者：
Pelser, Colleen;Arem, Hannah;Pfeiffer, Ruth M.;Elena, Joanne W.;Alfano, Catherine M.;Hollenbeck, Albert R.;Park, Yikyung
通讯作者：
Park, Yikyung