Hydrodynamic and diffusion considerations of rapid-mix experiments with red blood cells.

Hydrodynamic and diffusion considerations of rapid-mix experiments with red blood cells.
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红细胞快速混合实验的流体动力学和扩散考虑。

DOI:
10.1016/s0006-3495(80)85118-6
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发表时间:
1980
影响因子:
3.4
通讯作者:
Rice,SA
Rice,SA
中科院分区:
生物学3区
文献类型:
--
作者:
Rice,SA

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从使用快速混合技术对红细胞(RBC)的氧合速率的研究中,已经表明RBC被不(或不能)与其余水混合的停滞水层包围。适当的流体动力学和对流扩散速率的考虑表明,混合器可以将分辨时间减少到大约1 ms(或可能更少),并在TBC周围产生大约1微米厚的扩散层。在停流设备中,扩散层在大约10 ms内膨胀至大约4微米,而在连续流动工作中,扩散层膨胀的速度略慢且不太远。因此,当通过停流技术测量时,TBC的氧化速率应该比通过连续流装置测量时慢,对于连续流装置,速率将弱地依赖于询问管中的流的雷诺数。
From studies of the oxygenation rate of red blood cells (RBC) using rapid-mix techniques, it has been suggested that RBC are surrounded by a stagnant layer of water that does not (or cannot) mix with the rest of the water. A consideration of the appropriate hydrodynamics and convective diffusion rates shows that a mixer can reduce the resolution time to approximately 1 ms (or possibly less) and give a diffusion layer around the TBC that is approximately 1 micron thick. In stopped flow equipment it expands to approximately 4 micron over approximately 10 ms, whereas in continuous flow work the diffusion layers expands slightly less rapidly and less far. Thus the rate of oxygenation of TBC should be slower when measured by stopped flow techniques than by continuous flow apparatus for which the rate will depend weakly on the Reynolds number of the flow in the interrogation tube.
DOI: 10.1098/rspa.1923.0117
发表时间: 1923-10-01
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