Localization of caveolin-3 in the sinus endothelial cells of the rat spleen
Localization of caveolin-3 in the sinus endothelial cells of the rat spleen
复制标题
Caveolin-3 在大鼠脾窦内皮细胞中的定位
DOI：
10.1007/s00441-002-0516-0
复制
发表时间：
2002
影响因子：
3.6
通讯作者：
M. Miyoshi
中科院分区：
文献类型：
--
作者：
K. Uehara;M. Miyoshi
抽象的。共聚焦激光扫描和电子显微镜证实了小窝蛋白在大鼠脾窦内皮细胞中的定位。 Caveolin-3 是一种肌肉特异性的小窝蛋白，通过蛋白质印迹分析和免疫荧光显微镜对分离的窦内皮细胞和脾脏组织冷冻切片进行检测。在对分离的内皮细胞进行免疫荧光显微镜观察时，发现了caveolin-3和caveolin-1。在脾脏组织冷冻切片中，caveolin-3、caveolin-1 和 -2 存在于细胞的轮廓和细胞质中。组织冷冻切片的免疫金电子显微镜显示，caveolin-3、-1 和 -2 存在于顶端、外侧和基底质膜的小窝中，以及窦内皮细胞细胞质中的一些囊泡轮廓。此外，caveolin-3与caveolin-1共定位于顶质膜、侧质膜和基底质膜的相同小窝中。应力纤维和管状囊泡结构位于用抗-caveolin-3、抗-caveolin-1和抗-caveolin-2抗体标记的小窝附近。据推测，窦内皮细胞中的小窝在应力纤维的收缩中发挥重要作用。
Abstract. The localization of caveolins in the sinus endothelial cells of the rat spleen has been demonstrated by confocal laser scanning and electron microscopy. Caveolin-3, a muscle-specific caveolin, was detected by Western blot analysis and immunofluorescence microscopy of isolated sinus endothelial cells and tissue cryosections of the spleen. During the immunofluorescence microscopy of isolated endothelial cells, both caveolin-3 and caveolin-1 were found. In tissue cryosections of the spleen, caveolin-3, as well as caveolin-1 and -2, was present in the contours and cytoplasm of the cells. Immunogold electron microscopy of tissue cryosections revealed caveolin-3, -1, and -2 to be present in caveolae in the apical, lateral, and basal plasma membranes and some vesicular profiles in the cytoplasm of sinus endothelial cells. Furthermore, caveolin-3 was colocalized with caveolin-1 in the same caveolae in the apical, lateral, and basal plasma membranes. Stress fibers and tubulovesicular structures were situated in the vicinity of caveolae labeled with anti-caveolin-3, anti-caveolin-1, and anti-caveolin-2 antibodies. It is speculated that caveolae in sinus endothelial cells play an important role in the constriction of stress fibers.
DOI：
10.1073/pnas.93.1.131
发表时间：
1996-01-09
影响因子：
11.1
作者：
Scherer, PE;Okamoto, T;Lisanti, MP
通讯作者：
Lisanti, MP