Symbiosis preservation: Putative regulation of fatty acyl-CoA reductase by miR-31a within the symbiont harboring bacteriome through tsetse evolution.
Symbiosis preservation: Putative regulation of fatty acyl-CoA reductase by miR-31a within the symbiont harboring bacteriome through tsetse evolution.
复制标题
DOI：
10.3389/fmicb.2023.1151319
复制
发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
采采蝇是非洲锥虫的唯一媒介。除了锥虫外，采采蝇还携带着对采采蝇生物学至关重要的专性Wiggleschiagrossinidia细菌。没有Wigglesophoria导致苍蝇不育，从而为种群控制策略提供了希望。在这里，对两种进化上遥远的舌蝇物种（短须舌蝇和舌蝇）雌性中含有Wigglesworthia的独特细菌组和邻近的脱共生组织之间的微小RNA（miRNA）和mRNA表达进行了表征和比较。morsitans）。两个物种中共有193种miRNA表达，其中188种在两个物种中表达，其中166种是舌鱼科新发现的，41种miRNA在物种之间表现出相当的表达水平。在细菌体内，83个同源mRNA在G. morsitans aposybiotic和细菌组组织，其中21个具有保守的种间表达。这些差异表达的基因中有很大一部分参与氨基酸代谢和转运，象征着共生关系中必不可少的营养作用。进一步的生物信息学分析鉴定了细菌组内唯一保守的miRNA：：mRNA相互作用（miR-31 a：：脂肪酰辅酶A还原酶），其可能催化脂肪酸还原为醇，所述醇包含参与结构维持的酯和脂质组分。Glossina脂肪酰辅酶A还原酶基因家族的特点是通过系统发育分析，以进一步了解其进化的多样性和成员的功能作用。进一步研究miR-31 a：：脂肪酰辅酶A还原酶相互作用的性质，可能会发现新的贡献的共生利用载体控制。
Tsetse flies are the sole vectors of African trypanosomes. In addition to trypanosomes, tsetse harbor obligate Wigglesworthia glossinidia bacteria that are essential to tsetse biology. The absence of Wigglesworthia results in fly sterility, thus offering promise for population control strategies. Here, microRNA (miRNAs) and mRNA expression are characterized and compared between the exclusive Wigglesworthia-containing bacteriome and adjacent aposymbiotic tissue in females of two evolutionarily distant tsetse species (Glossina brevipalpis and G. morsitans). A total of 193 miRNAs were expressed in either species, with 188 of these expressed in both species, 166 of these were novel to Glossinidae, and 41 miRNAs exhibited comparable expression levels between species. Within bacteriomes, 83 homologous mRNAs demonstrated differential expression between G. morsitans aposymbiotic and bacteriome tissues, with 21 of these having conserved interspecific expression. A large proportion of these differentially expressed genes are involved in amino acid metabolism and transport, symbolizing the essential nutritional role of the symbiosis. Further bioinformatic analyses identified a sole conserved miRNA::mRNA interaction (miR-31a::fatty acyl-CoA reductase) within bacteriomes likely catalyzing the reduction of fatty acids to alcohols which comprise components of esters and lipids involved in structural maintenance. The Glossina fatty acyl-CoA reductase gene family is characterized here through phylogenetic analyses to further understand its evolutionary diversification and the functional roles of members. Further research to characterize the nature of the miR-31a::fatty acyl-CoA reductase interaction may find novel contributions to the symbiosis to be exploited for vector control.
影响因子：
5.2
作者：
Meki IK;İnce İA;Kariithi HM;Boucias DG;Ozcan O;Parker AG;Vlak JM;van Oers MM;Abd-Alla AMM
通讯作者：
Abd-Alla AMM