Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus).
Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus).
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DOI：
10.3389/fmicb.2018.02037
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发表时间：
2018
影响因子：
5.2
通讯作者：
Abd-Alla AMM
中科院分区：
文献类型：
--
作者：
Meki IK;İnce İA;Kariithi HM;Boucias DG;Ozcan O;Parker AG;Vlak JM;van Oers MM;Abd-Alla AMM
苍白舌唾液腺肥大病毒 (GpSGHV) 感染采采蝇主要无症状，偶尔有症状。有症状感染的特点是大规模饲养的采采蝇明显唾液腺肥大（SGH），这会导致生殖功能障碍和群体崩溃，从而阻碍通过昆虫不育技术（SIT）控制采采蝇。无症状感染对果蝇的健康没有明显的影响。在这里，对无症状和有症状感染的 G. pallidipes 果蝇的小 RNA 进行了测序和分析。无症状果蝇和有症状果蝇中均存在 38 个宿主编码的 microRNA (miRNA)，其中 9 个宿主 miRNA 在无症状果蝇中特异表达，而在有症状果蝇中则有 10 个。在共有的 38 个 miRNA 中，当比较无症状果蝇和有症状果蝇时，有 15 个存在差异表达。预测有症状果蝇中上调最多的宿主 miRNA 会靶向宿主的免疫相关 mRNA。鉴定出 6 个 GpSGHV 编码的 miRNA，其中 5 个仅存在于有症状的果蝇中。这些病毒编码的 miRNA 可能不仅针对宿主免疫基因，还可能参与病毒免疫逃避。有症状的果蝇 Glossina 中宿主 miRNA 谱差异的证据增进了我们对 GpSGHV-Glossina 相互作用的理解，并提供了潜在的新途径，例如通过利用特定的 miRNA 抑制剂或模拟物来更好地管理采采蝇大规模饲养设施中的 GpSGHV 感染，这是成功实施 SIT 的先决条件。
The Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infects tsetse flies predominantly asymptomatically and occasionally symptomatically. Symptomatic infections are characterized by overt salivary gland hypertrophy (SGH) in mass reared tsetse flies, which causes reproductive dysfunctions and colony collapse, thus hindering tsetse control via sterile insect technique (SIT). Asymptomatic infections have no apparent cost to the fly’s fitness. Here, small RNAs were sequenced and profiles in asymptomatically and symptomatically infected G. pallidipes flies determined. Thirty-eight host-encoded microRNAs (miRNAs) were present in both the asymptomatic and symptomatic fly profiles, while nine host miRNAs were expressed specifically in asymptomatic flies versus 10 in symptomatic flies. Of the shared 38 miRNAs, 15 were differentially expressed when comparing asymptomatic with symptomatic flies. The most up-regulated host miRNAs in symptomatic flies was predicted to target immune-related mRNAs of the host. Six GpSGHV-encoded miRNAs were identified, of which five of them were only in symptomatic flies. These virus-encoded miRNAs may not only target host immune genes but may also participate in viral immune evasion. This evidence of differential host miRNA profile in Glossina in symptomatic flies advances our understanding of the GpSGHV-Glossina interactions and provides potential new avenues, for instance by utilization of particular miRNA inhibitors or mimics to better manage GpSGHV infections in tsetse mass-rearing facilities, a prerequisite for successful SIT implementation.
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影响因子：
64.8
作者：
Cullen, Bryan R.
通讯作者：
Cullen, Bryan R.
影响因子：
3.8
作者：
Abd-Alla AM;Parker AG;Vreysen MJ;Bergoin M
通讯作者：
Bergoin M
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
6.4
作者：
De Vooght, Linda;Caljon, Guy;Van den Abbeele, Jan
通讯作者：
Van den Abbeele, Jan
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M