Progresses and challenges in Strongyloides spp. proteomics.
Progresses and challenges in Strongyloides spp. proteomics.
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拟圆线虫研究进展与挑战。蛋白质组学。
DOI：
10.1098/rstb.2022.0447
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发表时间：
2024-01-15
影响因子：
6.3
通讯作者：
Buonfrate, Dora
中科院分区：
文献类型：
--
作者：
Tiberti, Natalia;Manfredi, Marcello;Piubelli, Chiara;Buonfrate, Dora
过去二十年来提供的蠕虫基因组高质量数据的可用性支持并加速了大规模的组学研究，从而实现了对一些病原体的更深入的分子表征。这也涉及类圆线虫属。并且自从它们的基因组可用以来，转录组学已经相当频繁地应用于研究它们整个生命周期中的基因表达调控。类圆线虫蛋白质组学表征在某种程度上被忽视了，只有少数报告进行高通量或有针对性的分析与蛋白质鉴定的串联质谱。然而，为了辨别与人类类圆线虫病相关的重要方面，包括了解寄生虫生物学和宿主-寄生虫相互作用的机制，以及确定新的诊断和治疗靶点，这些调查是必要的。在这篇综述文章中，我们将给出一个概述已发表的蛋白质组学研究调查类圆线虫病在不同的水平，从不同的寄生虫阶段的体细胞蛋白质组和排泄/分泌产物的特性的调查潜在的免疫原性蛋白质。此外，为了填补宿主蛋白质组学的空白，我们还将首次对人类类圆线虫病患者进行血清蛋白质组学分析。这篇文章是Theo Murphy会议议题“类圆线虫：无虫种群的组学”的一部分。
The availability of high-quality data of helminth genomes provided over the past two decades has supported and accelerated large-scale ‘omics studies and, consequently, the achievement of a more in-depth molecular characterization of a number of pathogens. This has also involved Strongyloides spp. and since their genome was made available transcriptomics has been rather frequently applied to investigate gene expression regulation across their life cycle. Strongyloides proteomics characterization has instead been somehow neglected, with only a few reports performing high-throughput or targeted analyses associated with protein identification by tandem mass spectrometry. Such investigations are however necessary in order to discern important aspects associated with human strongyloidiasis, including understanding parasite biology and the mechanisms of host–parasite interaction, but also to identify novel diagnostic and therapeutic targets. In this review article, we will give an overview of the published proteomics studies investigating strongyloidiasis at different levels, spanning from the characterization of the somatic proteome and excretory/secretory products of different parasite stages to the investigation of potentially immunogenic proteins. Moreover, in the effort to try to start filling the current gap in host-proteomics, we will also present the first serum proteomics analysis in patients suffering from human strongyloidiasis. This article is part of the Theo Murphy meeting issue ‘Strongyloides: omics to worm-free populations’.
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DOI：
10.4049/jimmunol.1500745
发表时间：
2015-09-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Anuradha R;Munisankar S;Dolla C;Kumaran P;Nutman TB;Babu S
通讯作者：
Babu S
影响因子：
1.5
作者：
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通讯作者：
Skelly, Patrick J.
影响因子：
3.8
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DOI：
10.1111/j.1742-4658.2011.08248.x
发表时间：
2011-09
期刊：
The FEBS journal
影响因子：
--
作者：
Younis AE;Geisinger F;Ajonina-Ekoti I;Soblik H;Steen H;Mitreva M;Erttmann KD;Perbandt M;Liebau E;Brattig NW
通讯作者：
Brattig NW
影响因子：
5.7
作者：
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通讯作者：
Yang K