Proteomics Investigations of Potential Protein Biomarkers in Sera of Rabbits Infected With Schistosoma japonicum.
Proteomics Investigations of Potential Protein Biomarkers in Sera of Rabbits Infected With Schistosoma japonicum.
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DOI：
10.3389/fcimb.2021.784279
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发表时间：
2021
影响因子：
5.7
通讯作者：
Yang K
中科院分区：
文献类型：
--
作者：
Bi NN;Zhao S;Zhang JF;Cheng Y;Zuo CY;Yang GL;Yang K
血吸虫病是一种慢性寄生虫病，在许多发展中国家仍然是一个紧迫的公共卫生问题。该病的主要病理损害是虫卵聚集引起的肉芽肿和纤维化，早期治疗可有效预防肝纤维化的发生。因此，寻找可用于日本血吸虫感染早期诊断的生物标志物具有重要意义。本研究采用无标记蛋白质组学方法，观察了感染前、感染后1周和6周、治疗后5周和7周蛋白质的变化。总共有10种来自S.鉴定并定量了242种宿主来源的蛋白质，其变化显著。进行时间分析以进一步分析在感染和治疗期间具有连贯变化的潜在生物标志物。结果显示，与感染组和治疗组相比，血清蛋白的生物学过程发生变化，涉及受体介导的内吞作用、炎症反应和急性期反应，如甘露聚糖结合凝集素丝氨酸肽酶1、免疫球蛋白和胶原蛋白。这些发现为深入分析血吸虫病的潜在生物标志物、宿主蛋白和早期诊断提供了指导。及其致病机理数据可通过ProteomeXchange获得，标识符为PXD 029635。
Schistosomiasis is a chronic parasitic disease that continues to be a pressing public health problem in many developing countries. The primary pathological damage from the disease is granuloma and fibrosis caused by egg aggregation, and early treatment can effectively prevent the occurrence of liver fibrosis. Therefore, it is very important to identify biomarkers that can be used for early diagnosis of Schistosoma japonicum infection. In this study, a label-free proteomics method was performed to observe the alteration of proteins before infection, 1 and 6 weeks after infection, and 5 and 7 weeks after treatment. A total of 10 proteins derived from S. japonicum and 242 host-derived proteins were identified and quantified as significantly changed. Temporal analysis was carried out to further analyze potential biomarkers with coherent changes during infection and treatment. The results revealed biological process changes in serum proteins compared to infection and treatment groups, which implicated receptor-mediated endocytosis, inflammatory response, and acute-phase response such as mannan-binding lectin serine peptidase 1, immunoglobulin, and collagen. These findings offer guidance for the in-depth analysis of potential biomarkers of schistosomiasis, host protein, and early diagnosis of S. japonicum and its pathogenesis. Data are available via ProteomeXchange with identifier PXD029635.
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影响因子：
9.6
作者：
Batugedara G;Lu XM;Bunnik EM;Le Roch KG
通讯作者：
Le Roch KG
影响因子：
1.5
作者：
Carson JP;Robinson MW;Hsieh MH;Cody J;Le L;You H;McManus DP;Gobert GN
通讯作者：
Gobert GN
基于 iTRAQ 的日本血吸虫雌虫单性感染和双性感染的蛋白质组谱比较分析
影响因子：
3.3
作者：
Li, Xiaochun;Qiao, Hongbin;Jin, Yamei
通讯作者：
Jin, Yamei
DOI：
10.1038/nrg.2018.4
发表时间：
2018-05
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
Karczewski KJ;Snyder MP
通讯作者：
Snyder MP
影响因子：
3.3
作者：
Chen, Jun-Hu;Zhang, Ting;Zhou, Xiao-Nong
通讯作者：
Zhou, Xiao-Nong