Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.).
Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.).
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DOI：
10.3389/fpls.2018.01282
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发表时间：
2018
影响因子：
5.6
通讯作者：
Li A
中科院分区：
文献类型：
--
作者：
Zhao J;Zhai Z;Li Y;Geng S;Song G;Guan J;Jia M;Wang F;Sun G;Feng N;Kong X;Chen L;Mao L;Li A
TCP家族基因是植物特异性转录因子，在植物发育过程中起着重要作用。在一些植物中对tcp进行了进化和功能研究。虽然普通小麦（Triticum aestivum L.）是世界上主要的主要作物，但尚未对这种重要作物的tcp进行系统分析。在此，我们对小麦进行了全基因组调查，发现66个TCP基因属于22个同源群。然后，我们将这些基因定位到小麦染色体上，发现几个TCP基因在小麦中重复，包括玉米TEOSINTE BRANCHED 1的同源基因。RT-PCR和原位杂交研究表明，大部分TCP基因在小麦幼穗和未成熟种子发育过程中均有表达。顺式作用元件沿启动子区域的调查表明，同源基因可能发生了亚功能化。此外，三种TCP蛋白的蛋白相互作用实验表明，它们既可以形成同型二聚体，也可以形成异源二聚体。最后，我们鉴定了来自四倍体小麦的两个TaTCP9突变体。这两个突变系在a亚基因组同系物中都含有一个过早终止密码子，该密码子在B亚基因组同系物中主要表达。我们观察到突变导致穗长和粒长增加。总之，我们对小麦TCP基因家族的分析为进一步研究这些重要转录因子在小麦中的功能提供了一个起点。
The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this important crop has been conducted. Here, we performed a genome-wide survey in wheat and found 66 TCP genes that belonged to 22 homoeologous groups. We then mapped these genes on wheat chromosomes and found that several TCP genes were duplicated in wheat including the ortholog of the maize TEOSINTE BRANCHED 1. Expression study using both RT-PCR and in situ hybridization assay showed that most wheat TCP genes were expressed throughout development of young spike and immature seed. Cis-acting element survey along promoter regions suggests that subfunctionalization may have occurred for homoeologous genes. Moreover, protein–protein interaction experiments of three TCP proteins showed that they can form either homodimers or heterodimers. Finally, we characterized two TaTCP9 mutants from tetraploid wheat. Each of these two mutant lines contained a premature stop codon in the A subgenome homoeolog that was dominantly expressed over the B subgenome homoeolog. We observed that mutation caused increased spike and grain lengths. Together, our analysis of the wheat TCP gene family provides a start point for further functional study of these important transcription factors in wheat.
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影响因子：
7.2
作者：
ANDERSON, SL;TEAKLE, GR;KAY, SA
通讯作者：
KAY, SA
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Danisman S
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Danisman S
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通讯作者：
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