Overexpression of the leucine-rich receptor-like kinase gene LRK2 increases drought tolerance and tiller number in rice.
Overexpression of the leucine-rich receptor-like kinase gene LRK2 increases drought tolerance and tiller number in rice.
复制标题
富含亮氨酸受体样激酶基因 LRK2 的过度表达可提高水稻的耐旱性和分蘖数
DOI：
10.1111/pbi.12707
复制
发表时间：
2017-09
影响因子：
13.8
通讯作者：
Zha X
中科院分区：
文献类型：
--
作者：
Kang J;Li J;Gao S;Tian C;Zha X
干旱是全球作物分布的一个关键限制因素。受体样激酶在植物发育和对干旱等胁迫的防御反应中起着重要作用。在这项研究中，LRK 2，编码一个富含亮氨酸的受体样激酶，被克隆和表征，并发现定位在水稻的质膜上。启动子-GUS分析表明，在分蘖芽，根，节和花药中的强表达。与野生型相比，过表达LRK 2的转基因植物在营养生长阶段由于侧根数量的增加而表现出对干旱胁迫的耐受性增强。此外，LRK 2幼苗的异位表达导致分蘖发育增加。酵母双杂交筛选和双分子荧光互补（BiFC）表明LRK 2和延伸因子1 α（OsEF 1A）之间可能存在体外相互作用。这些结果表明，LRK 2通过增加水稻分支的发育，作为干旱胁迫反应和分蘖发育的正调节因子发挥作用。这些发现将有助于我们理解其他禾本科植物的分支调控，并支持水稻遗传学的改进。
Drought represents a key limiting factor of global crop distribution. Receptor‐like kinases play major roles in plant development and defence responses against stresses such as drought. In this study, LRK2, which encodes a leucine‐rich receptor‐like kinase, was cloned and characterized and found to be localized on the plasma membrane in rice. Promoter–GUS analysis revealed strong expression in tiller buds, roots, nodes and anthers. Transgenic plants overexpressing LRK2 exhibited enhanced tolerance to drought stress due to an increased number of lateral roots compared with the wild type at the vegetative stage. Moreover, ectopic expression of LRK2 seedlings resulted in increased tiller development. Yeast two‐hybrid screening and bimolecular fluorescence complementation (BiFC) indicated a possible interaction between LRK2 and elongation factor 1 alpha (OsEF1A) in vitro. These results suggest that LRK2 functions as a positive regulator of the drought stress response and tiller development via increased branch development in rice. These findings will aid our understanding of branch regulation in other grasses and support improvements in rice genetics.
登录
查看更多内容
影响因子：
7.2
作者：
Jiang, Yingnan;Chen, Xiuhua;Song, Wen-Yuan
通讯作者：
Song, Wen-Yuan
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
11.6
作者：
Ladwig, Friederike;Dahlke, Renate I.;Sauter, Margret
通讯作者：
Sauter, Margret
影响因子：
20.5
作者：
Chinchilla, Delphine;Shan, Libo;He, Ping;de Vries, Sacco;Kemmerling, Birgit
通讯作者：
Kemmerling, Birgit
影响因子：
11.2
作者：
Byun, Hae-Ok;Han, Na-Kyung;Lee, Jae-Seon
通讯作者：
Lee, Jae-Seon