Transcriptional signatures in iPSC-derived neurons are reproducible across labs when differentiation protocols are closely matched.
Transcriptional signatures in iPSC-derived neurons are reproducible across labs when differentiation protocols are closely matched.
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DOI：
10.1016/j.scr.2021.102558
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发表时间：
2021-10
影响因子：
1.2
通讯作者：
Cookson MR
中科院分区：
文献类型：
--
作者：
Reed X;Cobb MM;Skinbinski G;Roosen D;Kaganovich A;Ding J;Finkbeiner S;Cookson MR
来自不同实验室的ipsc衍生细胞表达模式的可重复性是确保在不同实验室进行的生化或功能分析复制的重要第一步。在这里，我们展示了在两个不同的实验室位置成熟和收集的ipsc和ipsc衍生神经元的可复制基因表达模式可以通过紧密匹配的协议和试剂实现。虽然iPSCs与分化神经元之间以及同一细胞类型的不同供体系之间的基因表达存在显著差异，但随实验室位点变化的转录变化相对较小。这些结果表明，在实验室之间努力匹配方案、试剂和技术方法可能会提高ipsc衍生细胞模型的可重复性。
Reproducibility of expression patterns in iPSC-derived cells from different labs is an important first step in ensuring replication of biochemical or functional assays that are performed in different labs. Here we show that reproducible gene expression patterns from iPSCs and iPSC-derived neurons matured and collected at two separate laboratory locations can be achieved by closely matching protocols and reagents. While there are significant differences in gene expression between iPSCs and differentiated neurons, as well as between different donor lines of the same cell type, transcriptional changes that vary with laboratory sites are relatively small. These results suggest that making great efforts to match protocols, reagents and technical methods between labs may improve the reproducibility of iPSC-derived cell models.
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DOI：
10.1016/j.mcn.2013.07.007
发表时间：
2013-09
期刊：
Molecular and cellular neurosciences
影响因子：
--
作者：
Burkhardt MF;Martinez FJ;Wright S;Ramos C;Volfson D;Mason M;Garnes J;Dang V;Lievers J;Shoukat-Mumtaz U;Martinez R;Gai H;Blake R;Vaisberg E;Grskovic M;Johnson C;Irion S;Bright J;Cooper B;Nguyen L;Griswold-Prenner I;Javaherian A
通讯作者：
Javaherian A
影响因子：
11
作者：
Soliman MA;Aboharb F;Zeltner N;Studer L
通讯作者：
Studer L
影响因子：
46.9
作者：
Risso, Davide;Ngai, John;Speed, Terence P.;Dudoit, Sandrine
通讯作者：
Dudoit, Sandrine
影响因子：
5.9
作者：
Volpato V;Smith J;Sandor C;Ried JS;Baud A;Handel A;Newey SE;Wessely F;Attar M;Whiteley E;Chintawar S;Verheyen A;Barta T;Lako M;Armstrong L;Muschet C;Artati A;Cusulin C;Christensen K;Patsch C;Sharma E;Nicod J;Brownjohn P;Stubbs V;Heywood WE;Gissen P;De Filippis R;Janssen K;Reinhardt P;Adamski J;Royaux I;Peeters PJ;Terstappen GC;Graf M;Livesey FJ;Akerman CJ;Mills K;Bowden R;Nicholson G;Webber C;Cader MZ;Lakics V
通讯作者：
Lakics V
影响因子：
25
作者：
HD iPSC Consortium
通讯作者：
HD iPSC Consortium