Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model.
Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model.
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DOI：
10.1186/gm299
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发表时间：
2011-12-29
期刊：
影响因子：
12.3
通讯作者：
Zon LI
中科院分区：
文献类型：
--
作者：
Martin CS;Moriyama A;Zon LI
斑马鱼模型在发育、造血和疾病的研究中正迅速获得重视。斑马鱼通过产生透明的外部受精胚胎，在早期胚胎发育过程中提供了比其他脊椎动物模型明显的优势。胚胎斑马鱼很容易可视化和操作，通过显微注射，化学处理和诱变。这些程序有助于大规模的化学，抑制和遗传筛选，以确定造血基因突变。人类和斑马鱼基因组之间的基因组保守性和局部同线性使得这些突变的基因组规模和表观遗传分析（通过微阵列、染色质免疫沉淀测序和RNA测序程序）成为转化研究和医学发现的有力方法。此外，大规模筛选技术已经鉴定出几种能够挽救造血缺陷和抑制疾病的小分子。在这里，我们讨论了斑马鱼模型的造血，造血干细胞发育和疾病相关的发现的理解的贡献。我们还强调了最近发现的具有临床前景的小分子，如二甲基前列腺素E2，3F8和噻唑甲酰胺10A。
The zebrafish model is rapidly gaining prominence in the study of development, hematopoiesis, and disease. The zebrafish provides distinct advantages over other vertebrate models during early embryonic development by producing transparent, externally fertilized embryos. Embryonic zebrafish are easily visualized and manipulated through microinjection, chemical treatment, and mutagenesis. These procedures have contributed to large-scale chemical, suppressor, and genetic screens to identify hematopoietic gene mutations. Genomic conservation and local synteny between the human and zebrafish genomes make genome-scale and epigenetic analysis of these mutations (by microarray, chromatin immunoprecipitation sequencing, and RNA sequencing procedures) powerful methods for translational research and medical discovery. In addition, large-scale screening techniques have resulted in the identification of several small molecules capable of rescuing hematopoietic defects and inhibiting disease. Here, we discuss the contributions of the zebrafish model to the understanding of hematopoiesis, hematopoietic stem cell development, and disease-related discovery. We also highlight the recent discovery of small molecules with clinical promise, such as dimethyl prostaglandin E2, 3F8, and thiazole-carboxamide 10A.
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