Biofilm formation by virulent and non-virulent strains of Haemophilus parasuis.
Biofilm formation by virulent and non-virulent strains of Haemophilus parasuis.
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DOI：
10.1186/s13567-014-0104-9
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发表时间：
2014-11-27
影响因子：
4.4
通讯作者：
Jacques M
中科院分区：
文献类型：
--
作者：
Bello-Ortí B;Deslandes V;Tremblay YD;Labrie J;Howell KJ;Tucker AW;Maskell DJ;Aragon V;Jacques M
副猪嗜血杆菌是一种寄生于健康猪上呼吸道的细菌。它也是Glässer病的病原体，Glässer病是一种全身性疾病，其特征为多关节炎、化脓性多浆膜炎和脑膜炎，导致仔猪的高发病率和死亡率。本研究的目的是评价生物膜形成的特点，强毒和无毒菌株的H。副猪。我们观察到，从健康猪的鼻腔中分离的无毒菌株形成的生物膜显著（p < 0.05）多于从患有Glässer病的猪的病变中分离的强毒株。当在静态条件下在微量滴定板中形成生物膜或在滴流装置或微流体系统中存在剪切力的情况下形成生物膜时，观察到这些差异。共聚焦激光扫描显微镜使用不同的荧光探针的代表性子集的菌株表明，生物膜基质含有聚-N-乙酰葡糖胺，蛋白质和eDNA。生物膜基质对蛋白酶K的降解高度敏感。非强毒H.副猪链球菌F9菌株的生物膜中发现了大量上调的膜相关基因，以及先前在胸膜肺炎放线杆菌生物膜中鉴定的基因。我们的数据表明，H。副猪链球菌与毒性、系统性菌株相比具有形成坚固生物膜的能力。因此，生物膜的形成可能允许无毒菌株在猪的上呼吸道中定殖并持续存在。相反，强毒株的非致病状态可能允许它们在宿主内传播。本文的在线版本（doi：10.1186/s13567-014-0104-9）包含补充材料，可供授权用户使用。
Haemophilus parasuis is a commensal bacterium of the upper respiratory tract of healthy pigs. It is also the etiological agent of Glässer’s disease, a systemic disease characterized by polyarthritis, fibrinous polyserositis and meningitis, which causes high morbidity and mortality in piglets. The aim of this study was to evaluate biofilm formation by well-characterized virulent and non-virulent strains of H. parasuis. We observed that non-virulent strains isolated from the nasal cavities of healthy pigs formed significantly (p < 0.05) more biofilms than virulent strains isolated from lesions of pigs with Glässer’s disease. These differences were observed when biofilms were formed in microtiter plates under static conditions or formed in the presence of shear force in a drip-flow apparatus or a microfluidic system. Confocal laser scanning microscopy using different fluorescent probes on a representative subset of strains indicated that the biofilm matrix contains poly-N-acetylglucosamine, proteins and eDNA. The biofilm matrix was highly sensitive to degradation by proteinase K. Comparison of transcriptional profiles of biofilm and planktonic cells of the non-virulent H. parasuis F9 strain revealed a significant number of up-regulated membrane-related genes in biofilms, and genes previously identified in Actinobacillus pleuropneumoniae biofilms. Our data indicate that non-virulent strains of H. parasuis have the ability to form robust biofilms in contrast to virulent, systemic strains. Biofilm formation might therefore allow the non-virulent strains to colonize and persist in the upper respiratory tract of pigs. Conversely, the planktonic state of the virulent strains might allow them to disseminate within the host. The online version of this article (doi:10.1186/s13567-014-0104-9) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Costa-Hurtado M;Ballester M;Galofré-Milà N;Darji A;Aragon V
通讯作者：
Aragon V
影响因子：
6.4
作者：
Blanchette-Cain K;Hinojosa CA;Akula Suresh Babu R;Lizcano A;Gonzalez-Juarbe N;Munoz-Almagro C;Sanchez CJ;Bergman MA;Orihuela CJ
通讯作者：
Orihuela CJ
影响因子：
4.4
作者：
Benoit, Michael R.;Conant, Carolyn G.;Matin, A.
通讯作者：
Matin, A.
影响因子：
3.2
作者：
Irie, Y;Mattoo, S;Yuk, MH
通讯作者：
Yuk, MH
影响因子：
4.4
作者：
Bouchet, Benedicte;Vanier, Ghyslaine;Gottschalk, Marcelo
通讯作者：
Gottschalk, Marcelo